PG

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• Code UNSPSC : 41106514

Propriétés

• Source biologique: fish ovary
• Conditionnement: tube of 5 μg 95070629-DNA-5UG; pkg of vial of cells 95070629-1VL
• Mode de croissance: Adherent
• Caryotype: Aneuploid
• Morphologie: Fibroblast-like
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Fish Northern Pike ovaries

Description de la lignée cellulaire

The fish cell line PG has been initiated 1976 from ovarian tissue of 5 juvenile pike (Esox lucius). Cells grow at 4°C to 25°C with an optimum temperature of 20°C. PG cells are susceptible to various fish viruses including infectious pancreatic necosis viruses and pike fry rhabdovirus. The cell line has been mycoplasma eradicated at ECACC.

Application

Fish Virology

Milieu de culture

MEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:3 to 1:6 using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 20°C Fish cell lines detach easily during transit if the culture is too young. After resuscitation split cells 1:3 to 1:4 and culture for at least one week wit

Autres remarques

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