DMS 79

95062824

• Code UNSPSC : 41106514

Propriétés

• Source biologique: human lung
• Mode de croissance: Aggregates in suspension
• Caryotype: Aneuploid
• Morphologie: Irregular
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human lung small cell carcinoma

Description de la lignée cellulaire

DMS79 has been established from pleural fluid of a patient with small cell carcinoma of the lung. The patient was treated with cytoxan, vincristine, methotrexate and radiation therapy. The cultures show a tendency for the modal chromosome number to decline. The production of adrenocorticotropin (ACTH), bombesin, calcitonin, β-endorphin, 17-β-estradiol, lipotropin, ocytocin-neurophysin, parathyroid hormone and somatstatin-like imuno-reactivity has been reported. The cells have also been reported to have the following cell surface antigens: Leu-7, Class I HLA, Class II HLA, Mg23 defined by the AML-2-23 monoclonal antibody and the AML-1-99 defined antigen. The cells have a receptor for epidermal growth factor (EGF) and produce tumours in nude athymic mice. Cells grow as irregularly shaped aggregates.

Application

Human lung tumour model, carcinogenesis studies.

Milieu de culture

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (Heat Inactivated).

Procédure de repiquage

Maintain cultures between 3 ? 9 x 100,000 cells/ml; 5% CO₂; 37°C. Only dilute 1:2 as a maximum. This cell line grows as clumps in suspension which can be easily dispersed with pipetting. Trypsinisation is not necessary. Routine splitting can therefore

Autres remarques

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