M. dunni (Clone III8C)

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 94101211, mouse tail, Fibroblast-like

• Code UNSPSC : 41106514

Propriétés

• Source biologique: mouse tail
• Conditionnement: tube of 5 μg 94101211-DNA-5UG; pkg of vial of cells 94101211-1VL
• Mode de croissance: Adherent
• Caryotype: Not specified
• Morphologie: Fibroblast-like
• Produits: Not specified
• Récepteurs: Ecotropic Murine leukemia virus (MuLV)
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Mouse tail, normal fibroblast

Description de la lignée cellulaire

Derived from the tail tissue of a normal female mouse, Mus dunni. The cells were cloned at passage 14 using Tererasiki plates. M. dunni (Clone III8C) supports the replication of all four classes of murine leukaemia viruses (MuLV); ecotropic, amphotropic, xenotropic and mink cell focus-forming viruses (MCF), showing cytopathic effect. The cells lack most endogenous murine leukaemia virus-related sequences. They are resistant to infection by Moloney MuLV, due to post-translational modifications of the ecotropic MuLV receptor. Confluent cultures will remain more firmly attached in RPMI 1640 or DMEM + 10% FBS during virus assays.

Application

Detection of replication-competent retroviruses in gene therapy.

Milieu de culture

McCoy′s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:3 to 1:8 i.e. seeding at 1-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Autres remarques

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