CHO-K1/SF

93061607, hamster ovary, Epithelial

• Code UNSPSC : 41106514

Propriétés

• product name: CHO-K1/SF, 93061607
• Source biologique: hamster ovary
• Mode de croissance: Adherent
• Caryotype: Not specified
• Morphologie: Epithelial
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Hamster Chinese ovary (MEM adapted)

Description de la lignée cellulaire

This MEM adapted sub-line of CHO-KI is used in Pertussis Toxin research and for quality control of batch production.

Application

CHO-K1/SF has been used to sequence the microRNA transcriptome and to identify, annotate and profile microRNAs as targets for cellular engineering. It has also been used to determine the pertussis toxin (PT) content of purified Bordetella pertussis fimbriae.

Milieu de culture

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Autres remarques

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.