C6 Cell Line from rat

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 92090409, rat nerve (glial tumor), Neuronal

• Synonyme(s) : C-6 Cells, RGC-6 Cells
• Code UNSPSC : 41106514

Propriétés

• Source biologique: rat nerve (glial tumor)
• Description: Rat glial tumor
• Conditionnement: tube of 5 μg 92090409-DNA-5UG; pkg of vial of cells 92090409-1VL
• Mode de croissance: Adherent
• Caryotype: 2n = 42
• Morphologie: Neuronal
• Produits: S-100 protein, somatotrophin
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Rat glial tumour

Description de la lignée cellulaire

Derived from a rat glial tumour induced by N-nitrosomethylurea. The cells produce S-100 protein which is found in the neural tissue of vertebrates and has been found in brain tumours. Synthesis of S-100 increases 10 fold as cells reach confluency.

Application

C6 cell line has been used to study the ability of 2-(2,4-dihydroxyphenyl)thieno-1,3-thiazin-4-one (BChTT) to inhibit cancer cell proliferation and identify mechanisms involved in its actions. It has also been used to study the cytotoxic and antioxidant properties of supercritical CO₂ extract (SCE) of old man′s beard (Usnea barbata).

C6 cells are reported to produce elevated levels of glyceryl phosphate dehydrogenase activity in response to glucocorticoids

Milieu de culture

Ham′s F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x100,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Autres remarques

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