70Z/3

91092002, mouse blood, Lymphoblastoid

• Code UNSPSC : 41106514

Propriétés

• product name: 70Z/3, 91092002
• Source biologique: mouse blood
• Mode de croissance: Suspension
• Caryotype: Not specified
• Morphologie: Lymphoblastoid
• Produits: Cytoplasmic Immunoglobulin
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Mouse pre-B lymphocyte

Description de la lignée cellulaire

Derived from a methyl nitrosourea-induced tumour in a (C57BL/6XDBA/2) F1 mouse. This line is positive for cytoplasmic Ig and negative for surface membrane Ig and TdT.

Milieu de culture

RPMI 1640 + 2mM Glutamine + 0.05mM 2-Mercaptoethanol (2ME) + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Maintain cultures between 2-5x100,000 cells/ml; 5% CO₂; 37°C.

Autres remarques

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Depositor and originator: Dr P Kincade, Oklahoma Medical Research Foundation