J774A.1 Cell Line from mouse

91051511, mouse blood, Not specified

• Synonyme(s) : J774 A1 Cells, J774.A1 Cells, J774A1 Cells
• Code UNSPSC : 41106514

Propriétés

• Nom du produit: J774A.1 Cell Line from mouse, 91051511
• Source biologique: mouse blood
• Description: Mouse BALB/c monocyte macrophage
• Mode de croissance: Semi-adherent
• Caryotype: Not specified
• Morphologie: Not specified
• Produits: Interleukin 1 (IL-1), lysozyme
• Récepteurs: Immunoglobulin, complement
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Mouse BALB/c monocyte macrophage

Description de la lignée cellulaire

Derived from a tumour in a female BALB/c mouse. Cell line produces IL-5 and lysozyme.

Application

J774A.1 cell line has been used to test the cytotoxicity of [1,2,3]triazolo[1,5-a]pyridinium salts (having leishmanicidal activity) from triazolopyridines. It has also been used to determine the antitrypanosomatid activity of flavonoid glycosides isolated from Delphinium sps.

Metabolic studies

Milieu de culture

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Cells are subcultured by harvesting attached and suspension cells. Remove majority of medium and centrifuge to harvest cells growing in suspension. Scrape adherent cells remaining in the flask. Resuspend cell pellet from centrifugation and combine with scraped cells. Seed into new flask at 1:3 to 1:4 split ratio i.e. seeding at 1-3 x 10,000 cells/cm² Incubate at 37°C; 5% CO₂. Please Note :- On receipt growing order cells may be in suspension. The cells and media should be removed from the flask and spun down to form a pellet. This should then be re-suspended in fresh media and a cell count performed. The cells should then be reseeded at 2-4 x 10E4/cm and should begin to show signs of re-attachment within 48 hours.

Autres remarques

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