MCA-RH 7777

90021504, rat liver, Hepatocyte

• Code UNSPSC : 41106514

Propriétés

• Nom du produit: MCA-RH 7777, 90021504
• Source biologique: rat liver
• Conditionnement: tube of 5 μg 90021504-DNA-5UG; pkg of vial of cells 90021504-1VL
• Mode de croissance: Adherent
• Caryotype: Not specified
• Morphologie: Hepatocyte
• Produits: Alpha-foetoprotein
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Rat Buffalo hepatoma

Description de la lignée cellulaire

Derived from the Morris hepatoma 7777 (obtained from a male Buffalo strain rat). The original tumour was induced with N-2 fluorenylphthalamic acid. Particularly interesting for oncogeny studies since the cells produce α-fetoprotein (AFP).

Application

Tumourigenicity and oncogeny studies

Milieu de culture

Swimm′s S-77 + 4mM Glutamine + 20% Horse Serum (HS) + 5% Foetal Bovine Serum (FBS) or EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 20% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split semi-confluent cultures 1:2 to 1:6 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C. NB: Cells are shed from the monolayer before confluency is achieved.

Autres remarques

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