CCRF-SB

89090405, human blood, Lymphoblastoid

• Code UNSPSC : 41106514

Propriétés

• product name: CCRF-SB, 89090405
• Source biologique: human blood
• Mode de croissance: Suspension
• Caryotype: 2n = 46
• Morphologie: Lymphoblastoid
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human Caucasian acute lymphoblastic leukaemia

Description de la lignée cellulaire

A B lymphoblastoid line obtained from the peripheral blood of an 11 year old boy with acute lymphoblastic leukaemia. Appear free of virus particles by electron microscopy. Do not synthesise immunoglobulins. Grown in continuous spinner culture for 6 months. HLA profile: A1, A2, B12, B17, Cw2.

Application

HLA profile studies

Milieu de culture

RPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

Procédure de repiquage

Maintain cultures between 3x10,000 to 1x100,000 cells/ml; 5% CO₂; 37°C. Cultures can tolerate high densities i.e. 1-2x1,000,000 cells/ml.

Autres remarques

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