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Key Documents

COLO 201

87091201, human colon. Clumps of spherical cells in suspension, cuboidal cells in monolayer.

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About This Item

Code UNSPSC :
41106514

product name

COLO 201, 87091201

Source biologique

human colon

Mode de croissance

Adherent

Caryotype

Triploid modal no. 71-78

Morphologie

Clumps of spherical cells in suspension, cuboidal cells in the monolayer

Produits

Carcinoembryonic antigen (CEA) less than produced by COLO 205

Récepteurs

Not specified

Technique(s)

cell culture | mammalian: suitable

Maladie(s) pertinente(s)

cancer

Conditions d'expédition

dry ice

Température de stockage

−196°C

Origine de la lignée cellulaire

Human Caucasian colon adenocarcinoma

Description de la lignée cellulaire

COLO 201, COLO 205 and COLO 206 (ECACC Catalogue 93052620) were all established from the ascites fluid obtained from a 70-year-old male patient with adenocarcinoma of the colon (Dukes Classification Grade D). At time of isolation, COLO 201 displayed cuboidal morphology but with increasing passage the depositor reported that COLO 201 tends to display predominantly rounded cells in clumps in suspension with cuboidal cells in the monolayer. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

Profil d'ADN

STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 10
D16S539: 12,13
D5S818: 10,13
D7S820: 9,10
THO1: 8,9
TPOX: 11
vWA: 15

Milieu de culture

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Maintain suspension cells at 3-9x105 cells/cm3. Split sub-confluent cultures (70-80%) of attached cells 1:3 to 1:6 i.e. seeding at 2-3x104 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.

Autres remarques

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Contenu apparenté

We offer mouse, rat, and 40+ human colorectal cancer cell lines including Caco-2 and HT29 for applications including drug delivery and treatment strategies, and metastasis studies. Choose cells with relevant mutations in genes such as KRAS, APC, TP53, and PTEN.

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