3T3 clone A31 Cell Line from mouse

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 86110401, mouse embryo, Fibroblast

• Synonyme(s) : 3T3-A31 Cells, BALB/3T3 clone A31 Cells, BALB/c 3T3 clone A31 Cells
• Code UNSPSC : 41106514

Propriétés

• Source biologique: mouse embryo
• Description: Mouse BALB/c embryo
• Conditionnement: tube of 5 μg 86110401-DNA-5UG; pkg of vial of cells 86110401-1VL
• Mode de croissance: Adherent
• Caryotype: 2n = 40, hypotetraploid
• Morphologie: Fibroblast
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Mouse BALB/c embryo

Description de la lignée cellulaire

Cells have now lost their contact inhibition.

Application

3T3 clone A31 cell line from mouse has been used:

• to study in vitro cytotoxicity and genotoxicity of retinyl palmitate–loaded poly(lactide-co-glycolide) nanoparticles for the topical treatment of skin diseases
• for the characterization of the phototoxicity of Vemurafenib, which is a B-Raf serine/threonine kinase inhibitor
• for the phototoxicity assessment of systemically applied compounds and drug candidates

Tumourigenicity and virus studies: SV40 and MSV

Milieu de culture

DMEM + 2mM Glutamine + 5% New Born Calf Serum (NBCS) + 5% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:5 to 1:20 i.e. seeding at 5x1,000 to 2x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Autres remarques

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