Hep2 (HeLa derivative) Cell Line human

86030501, human cervix, Epithelial

• Code UNSPSC : 41106514

Propriétés

• product name: Hep2 (HeLa derivative) Cell Line human, 86030501
• Source biologique: human cervix
• Description: Human Black, cervix carcinoma
• Mode de croissance: Adherent
• Caryotype: Modal no. 14
• Morphologie: Epithelial
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human cervix carcinoma

Description de la lignée cellulaire

Originated from tumours produced in irradiated-cortisonised weanling rats after injecting with epidermoid carcinoma tissue from the larynx of a 56 year old male. This cell line was found to be indistinguishable from HeLa by STR PCR DNA profiling. Therefore, the cell line should be considered as derived from HeLa. Ethnicity: Black.

Application

Human Hep2 (HeLa derivative) cell line has been used for virus and tumorigenicity studies.

Milieu de culture

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. After thawing the first subculture may take 7-10 days.

Autres remarques

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