111A3

85062504, Application: Fusion partner in the production of heterohybridomas

• Code UNSPSC : 41106514

Propriétés

• Source biologique: Mouse x Bovine Heterohybridoma
• ImmunoDon: Jersey calf lymph node
• Myélome: 53B3 (mouse x bovine heteromyeloma)
• Réactivité: Application: Fusion partner in the production of heterohybridomas
• Technique(s): cell culture | mammalian: suitable
• Isotype: Not specified
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Mouse 53B3 x Bovine Jersey Calf lymph node

Description de la lignée cellulaire

Non Ig secreting

Immunogène

Sheep red blood cells

Application

This line has been used as a HAT sensitive fusion partner in the production of heterohybridomas

Milieu de culture

RPMI 1640 + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 10% Horse Serum (HS)

Procédure de repiquage

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C

Autres remarques

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