VERO Cell Line

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 84113001, African green monkey kidney, Fibroblast-like

• Synonyme(s) : Vero 81 Cells, VeroCCL81 Cells
• Code UNSPSC : 41106514

Propriétés

• Source biologique: African green monkey kidney
• Description: Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)
• Conditionnement: tube of 5 μg 84113001-DNA-5UG; pkg of vial of cells 84113001-1VL
• Mode de croissance: Adherent
• Caryotype: 2n = 60, modal no. 58
• Morphologie: Fibroblast-like
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Description générale

Vero cells are anchorage-dependent cells and have wide applications in molecular and cell biology research. Vero cells have a pseudo-diploid karyotype and are preferred cell lines for the propagation of various viruses, including poliovirus, rotavirus influenza and rabies virus. These cells are useful hosts for vaccine production.

Origine de la lignée cellulaire

Monkey African Green kidney

Description de la lignée cellulaire

Established from the kidney of a normal adult African Green monkey. Susceptible to a wide range of viruses including polio, rubella, arboviruses and reoviruses.

Application

VERO cell lines have been used:

• in the propagation of Chandipura virus (CHPV)
• in the cytotoxicity assay to evaluate the antioxidant and anti-viral activities of Capsicum annuum methanolic extracts
• for the propagation of T.cruzi

Virus studies: polio, rubella, arboviruses and reoviruses

Milieu de culture

(D6546) + 2 mM (G7513) + 10% (F2442).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 °C.

Autres remarques

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