MRC-5 SV2

84100401, human lung (fetal), Fibroblast

• Synonyme(s) : MRC-5V2 Cells, MRC5V2 Cells
• Code UNSPSC : 41106514

Propriétés

• product name: MRC-5 SV2, 84100401
• Source biologique: human lung (fetal)
• Mode de croissance: Adherent
• Caryotype: Modal no. = 60-80, hyperdiploid
• Morphologie: Fibroblast
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human foetal lung, SV40 transformed

Description de la lignée cellulaire

Derived from the parental cell line MRC-5 by transformation with the SV40 virus. The cells do not senesce.

Application

Transformation and virus studies: SV40

Profil d'ADN

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11,14
D16S539: 11
D5S818: 11,12
D7S820: 10
THO1: 8
TPOX: 8
vWA: 15

Milieu de culture

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 1-2x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Autres remarques

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.