LIM2099 cell line human

12062002, human liver (metastasis), epithelial

• Synonyme(s) : LIM 2099, LIM-2099
• Code UNSPSC : 41106514
• Source biologique: human liver (metastasis)
• Mode de croissance: adherent
• Morphologie: epithelial

Propriétés

• Nom du produit: LIM2099 cell line human, 12062002
• Source biologique: human liver (metastasis)
• Mode de croissance: adherent
• Morphologie: epithelial
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Description de la lignée cellulaire

Human liver metastasis, moderately differentiated sclerosing adenocarcinoma. Cell Bank Australia reference number: CBA0164.

Milieu de culture

RPMI-1640 (with 2mM L-Glutamine + 25mM HEPES) + 10% FCS, Insulin 0.6 μg/ml, Hydrocortisone 1 μg/ml, 1-Thioglycerol 10μM

Procédure de repiquage

Optimal split ratio 1:4-1:6 (seeding density 0.8 x 10⁴ cells/cm²). Cells incubated at 37°C with 5% CO₂. Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5-10 min.

Autres remarques

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