HT29-MTX-E12

from human colon, 12040401

• Synonyme(s) : HT-29-MTX-E12 Cells, HT29, HT29 Cells, HT29-MTX, HT29-MTX Cells, MTX-E12 Cells
• Code UNSPSC : 41106514

Propriétés

• Source biologique: human colon
• Mode de croissance: Adherent
• Caryotype: Not specified
• Morphologie: Epithelial
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

HT29, HT29-MTX, methotrexate, mucous layer, intestinal permeation, oral drug absorption

Description de la lignée cellulaire

HT29 cells were differentiated into mature goblet cells using methotrexate. Mucous-secreting HT29-MTX subclones were isolated from this cell clone and characterized with regard to tight junction formation, development of confluent monolayers and productio

Profil d'ADN

STR-PCR Data:
Amelogenin: X
CSF1PO: 11,12
D13S317: 11
D16S539: 11,12
D5S818: 11,12
D7S820: 10
THO1: 6
TPOX: 8,9
vWA: 17

Milieu de culture

DMEM + 2mM Glutamine + 1% Non essential amino acids + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split subconfluent cultures (70-80%) 1:3 to 1:6 using 0.25% trypsin/EDTA; 7.5-10% CO_2; 37 °C. Suggested seeding density 2-4x10,000 cells/cm^².

Autres remarques

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