C6-BU-1

8062563, rat brain, fibroblastic/fusiform

• Code UNSPSC : 41106514

Propriétés

• Nom du produit: C6-BU-1, 08062563
• Source biologique: rat brain
• Conditionnement: tube of 5 μg 08062563-DNA-5UG; pkg of vial of cells 08062563-1VL
• Mode de croissance: Adherent
• Caryotype: modal No. of chromosomes is 42
• Morphologie: fibroblastic/fusiform
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Rat glioma x rat glioma hybrid

Description de la lignée cellulaire

A 8-bromodeoxybromidine resistant mutant of the rat glioma cell line C6. Found to be tumourigenic in BALB/c nude mice and Wistar rats. Strain rattus norvegicus

Application

Used in the generation of cell hybrids.

Milieu de culture

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 1mM 8-bromodeoxyuridine. The cells can be grown in the absence of bromodeoxyuridine.

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C

Autres remarques

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