PNT1A (SERUM FREE)

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 7052901, human prostate, Not specified

• Code UNSPSC : 41106514

Propriétés

• Source biologique: human prostate
• Conditionnement: tube of 5 μg 07052901-DNA-5UG; pkg of vial of cells 07052901-1VL
• Mode de croissance: Semi-adherent
• Caryotype: Not specified
• Morphologie: Not specified
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human post pubertal normal, immortalised with SV40, serum-free

Description de la lignée cellulaire

This cell line is a derivative of the normal human prostate cell line PNT1A (Sigma cat no. 95012614) adapted to grow in serum-free medium. PNT1A was established by immortalisation of normal adult prostatic epithelial cells by transfection with a plasmid containing SV40 genome with a defective replication origin. The primary culture was obtained from the prostate of a 35 year old male at post mortem.

Milieu de culture

Serum-free Stemline Keratinocyte Medium II (Sigma cat no. S0196) + Keratinocyte Medium Supplement (Sigma cat no. K3136). To freeze cells use 50:50 fresh:conditioned medium + 10% DMSO

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:2; 5% CO₂; 37 ºC. Mixture of attached and suspension cells. To harvest attached cells use gentle agitation. If cells do not detach, then try using 0.25% trypsin. A trypsin inhibitor must be used to inactivate trypsin. Seed contents of one ampoule into a 25 cm² flask.

Autres remarques

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