KARPAS 45

6072602, human bone marrow, Spherical cells

• Code UNSPSC : 41106514

Propriétés

• product name: KARPAS 45, 06072602
• Source biologique: human bone marrow
• Mode de croissance: Suspension
• Caryotype: Hypotetraploid
• Morphologie: Spherical cells
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human T-cell Leukaemia

Description de la lignée cellulaire

A human T cell leukaemia cell line established from the bone marrow of a 2-year-old boy diagnosed with acute lymphoblastic leukaemia (ALL) prior to therapy in 1972; cells were reported to carry the MLL-AFX and AFX-MLL fusion genes, the original diagnosis was T-ALL.

Milieu de culture

RPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS). For slower growth use 10% or 5% FBS.

Procédure de repiquage

Maintain cultures between 0.5 - 2 x1000,000 cells/ml; 5% CO₂; 37°C. Doubling time 50-100 hours. Split saturated culture 1:2 to 1:3 every 2-3 days. Spherical cells growing in clusters in suspension.

Autres remarques

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Clause de non-responsabilité

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.