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Key Documents

C5405

Sigma-Aldrich

Chicken Serum

USA origin, sterile-filtered, suitable for cell culture

Synonyme(s) :

chicken sera

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About This Item

Code UNSPSC :
12352207
Nomenclature NACRES :
NA.71

Source biologique

chicken

Stérilité

sterile-filtered

Forme

liquid

Composition

hemoglobin, ≤60 mg/dL

Origine

USA origin

Technique(s)

cell culture | mammalian: suitable

Impuretés

≤50 EU/mL endotoxin

Conditions d'expédition

dry ice

Température de stockage

−20°C

Application

Chicken Serum has been used:
  • for pellet resuspension following centrifugation, to prepare zymosan suspension for respiratory burst assay
  • as a supplement to Dulbecco′s modified Eagle′s medium (DMEM) to culture HeLa P cells
  • for blocking in immunofluorescence (IF) double-staining

Chicken serum is used in parasitology to study excystation, growth, survival and egg production of the microphallid trematode Maritrema novaezealandensis. Chicken serum is used for the serial cultivation of chicken keratinocytes.

Actions biochimiques/physiologiques

Animal sera serve as a cost-effective alternative to basal cell culture media supplement. They are commonly used in immunodetection protocols such as immunohistochemistry (IHC), immunocytochemistry (ICC), and western blot to block nonspecific binding. Chicken serum is suitable for use in parasitology to study excystation, growth, survival, and egg production of the microphallid trematode Maritrema novaezealandensis. It is used for the serial cultivation of chicken keratinocytes.

Remarque sur l'analyse

Endotoxin tested

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves

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Consulter la Bibliothèque de documents

Géraldine Rouquet et al.
Journal of bacteriology, 191(13), 4427-4440 (2009-04-21)
We identified a carbohydrate metabolic operon (frz) that is highly associated with extraintestinal pathogenic Escherichia coli (ExPEC) strains. The frz operon codes for three subunits of a phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS) transporter of the fructose subfamily, for a transcriptional activator
In vitro cultivation of Maritrema novaezealandensis (Microphallidae): the effect of culture medium on excystation, survival and egg production.
Fredensborg BL, Poulin R.
Parasitology Research, 95, 10-13 (2005)
Tom W Anderson et al.
Molecular biology of the cell, 19(11), 5006-5018 (2008-09-19)
In migrating fibroblasts actomyosin II bundles are graded polarity (GP) bundles, a distinct organization to stress fibers. GP bundles are important for powering cell migration, yet have an unknown mechanism of formation. Electron microscopy and the fate of photobleached marks
Amandine Vanhoutteghem et al.
Differentiation; research in biological diversity, 72(4), 123-137 (2004-05-26)
The epidermis of birds differs from that of mammals by the presence of intracellular lipid droplets and the absence of sebaceous glands. We describe here the cultivation of chicken epidermal keratinocytes; these cells cannot be grown in medium supplemented with
Marc A J Morgan et al.
PloS one, 8(2), e56738-e56738 (2013-02-19)
The spontaneous destruction of insulin producing pancreatic beta cells in non-obese diabetic (NOD) mice provides a valuable model of type 1 diabetes. As in humans, disease susceptibility is controlled by the classical MHC class II genes that guide CD4(+) T
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