C4407
Human Collagen Type III
from human placenta, powder, suitable for cell culture
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About This Item
Numéro CAS:
Numéro CE :
Numéro MDL:
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.32
Produits recommandés
product name
Collagène from human placenta, Bornstein and Traub Type III (Sigma Type X), powder
Source biologique
human placenta
Niveau de qualité
Pureté
≥90% (GE)
Forme
powder
Technique(s)
cell culture | mammalian: suitable
Impuretés
HIV, hepatitis B and hepatitis C, none detected
Solubilité
aqueous acid: soluble
Numéro d'accès UniProt
Température de stockage
2-8°C
Informations sur le gène
human ... COL3A1(1300)
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Application
Collagen from human placenta has been used to assess the bioelectric effects of quinine on airway epithelial cells, to study the selective toxicity of engineered lentvirus lytic peptides and in a particle aggregation assay for the rapid detection of fibronectin, fibrinogen and collagen receptors on Staphylococcus aureus. It has also been used in a magnesium-dependent, collagen-binding assay during characterization of human lung tumor-associated antigens.
Composants
All collagen molecules are composed of three polypeptide chains arranged in a triple helical conformation, with a primary structure that is mostly a repeating motif with glycine in every third position and proline or 4-hydroxyproline frequently preceding the glycine residue.
Attention
This product should be stored at 2-8 °C and will retain activity in these conditions for 2 years.
Notes préparatoires
This product was prepared by a modification of the pepsin extraction and salt fractionation method of Hill, R.J. and Harper, E., Anal. Biochem., 141 (1984). It is a lyophilized powder that can be reconstituted in sterile .5 M acetic acid, PBS or water at 1 mg/mL. A PBS solution will be stable for at least one year at -20°C.
Remarque sur l'analyse
An SDS polyacrylamide gel electrophoresis test run under reducing conditions consistent with basement membrane collagen yields three major bands.
Autres remarques
Le collagène se classe en un certain nombre de types structurellement et génétiquement distincts. Nous utilisons la nomenclature proposée par Bornstein et Traub. Il est important de ne pas confondre les désignations utilisées par Sigma et les types de classification reconnus.
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
Certificats d'analyse (COA)
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