C0979
Anti-Catalase antibody ,Mouse monoclonal
clone CAT-505, purified from hybridoma cell culture
Synonyme(s) :
Anti-CAT
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About This Item
Produits recommandés
Source biologique
mouse
Conjugué
unconjugated
Forme d'anticorps
purified from hybridoma cell culture
Type de produit anticorps
primary antibodies
Clone
CAT-505, monoclonal
Forme
buffered aqueous solution
Poids mol.
antigen 60 kDa
Espèces réactives
human, mouse, bovine, rat
Concentration
~2 mg/mL
Technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
indirect ELISA: suitable
microarray: suitable
western blot: 0.5-1 μg/mL using a whole extract of HepG2 cells
Isotype
IgG1
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Température de stockage
−20°C
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... CAT(847)
mouse ... Cat(12359)
rat ... Cat(24248)
Catégories apparentées
Description générale
Catalase is one of the most efficient and common enzyme found in nearly all living organisms exposed to oxygen. It is a very important enzyme in protecting the cell from oxidative damage by reactive oxygen species (ROS). In most mammalian cell types catalase is localized in peroxisomes which is a tertrameric haemin-enzyme consisting of 4 identical tetrahedrally arranged subunits of 60kDa. It contains four ferriprotoporphyrin groups per molecule having a molecular mass of 240kDa.
Immunogène
human erythrocyte catalase
Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (5 papers)
Western Blotting (5 papers)
Monoclonal Anti-Catalase antibody produced in mouse is suitable for the following:
- Immunocytochemistry
- Immunohistochemistry
- Indirect ELISA
- Microarray
- Western blotting at a concentration of 0.5-1μg/mL using whole extract of HepG2 cells
- Immunofluorescence
Actions biochimiques/physiologiques
Catalase reacts with hydrogen peroxide, a reactive oxygen species, activating its decomposition into water and molecular oxygen and it reacts with hydrogen donors. It functions as a natural anti-oxidant, protecting cells against oxidative damage of proteins, lipids and nucleic acids. Catalase also plays a role in gene expression and apoptosis. Antioxidants have been identified in association with a variety of diverse cellular functions including growth control, proliferation, differentiation, immune response, tumor promotion, apoptosis and activation of viruses.
Forme physique
Solution in 0.01 M phosphate buffered saline, pH7.4, containing 15 mM sodium azide
Notes préparatoires
Purified from culture supernatant of hybridoma cells grown in a bioreactor.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
nwg
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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Les clients ont également consulté
The curious case of peroxiredoxin-5: what its absence in aves can tell us and how it can be used
Pirson M, et al.
BMC Evolutionary Biology, 18(1), 18-18 (2018)
P Chelikani et al.
Cellular and molecular life sciences : CMLS, 61(2), 192-208 (2004-01-28)
More than 300 catalase sequences are now available, divided among monofunctional catalases (> 225), bifunctional catalase-peroxidases (> 50) and manganese-containing catalases (> 25). When combined with the recent appearance of crystal structures from at least two representatives from each of
K Aoshiba et al.
Journal of immunology (Baltimore, Md. : 1950), 162(3), 1692-1700 (1999-02-11)
Neutrophils constitutively undergo apoptosis at both normal and inflamed sites: an important process that limits the toxic potential of the neutrophil. However, the signal pathway for neutrophil apoptosis is currently unknown. In this study, we evaluated the role of p38-mitogen-activated
J J Whyte et al.
Transgenic research, 20(5), 989-1001 (2010-12-21)
The objective of this study was to develop transgenic Yucatan minipigs that overexpress human catalase (hCat) in an endothelial-specific manner. Catalase metabolizes hydrogen peroxide (H(2)O(2)), an important regulator of vascular tone that contributes to diseases such as atherosclerosis and preeclampsia.
J Kodydková et al.
Folia biologica, 60(4), 153-167 (2014-08-26)
Catalase (CAT) is a well-studied enzyme that plays an important role in protecting cells against the toxic effects of hydrogen peroxide. In human, it has been implicated in different physiological and pathological conditions. This review summarizes the information available on
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