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C0794

Sigma-Aldrich

Chitosanase from Streptomyces sp.

buffered aqueous glycerol solution, ≥15 units/mg protein (E1%)

Synonyme(s) :

Chitosan N-acetylglucosaminohydrolase

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About This Item

Numéro CAS:
51570-20-8
Numéro de classification (Commission des enzymes):
3.2.1.132 (BRENDA, IUBMB)
Numéro MDL:
MFCD00211797
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54

Forme

buffered aqueous glycerol solution

Activité spécifique

≥15 units/mg protein (E1%)

Concentration

≥0.5 mg/mL

Solubilité

0.1 M acetate buffer, pH 5.5: soluble

Température de stockage

−20°C

Application

Chitosanase from Sigma has been used for polarized light-stimulated, enzymatic hydrolysis of chitin and chitosan.

Actions biochimiques/physiologiques

Chitosanase catalyzes the endohydrolysis of β-1,4-linkages between D-glucosamine (GlcN-GlcN) residues in chitosan. The enzyme from Streptomyces has been reported to also hydrolyze the GlcNAc-GlcN linkage in partially acetylated chitosan.

Définition de l'unité

One unit will liberate 1.0 μmole of reducing sugar (measured as D-glucosamine equivalents) from chitosan per minute at pH 5.5 at 37 °C.

Forme physique

Solution in 50% glycerol containing 50 mM sodium acetate

Notes préparatoires

Soluble in 10mL of 0.1 M Acetate buffer at pH 5.5

Autres remarques

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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Anna Konieczna-Molenda et al.
Carbohydrate research, 343(18), 3117-3119 (2008-10-01)
Illumination with white linearly polarized light (WLPL) stimulated chitinase and chitosanase in their degradation of chitin and chitosan, respectively. Enzymes were illuminated at room temperature in separate vessels, then admixed in reactors containing polysaccharides. Hydrolysis of chitosan to glucosamine followed
A new reaction for colorimetric determination of carbohydrates.
M Lever
Analytical biochemistry, 47(1), 273-279 (1972-05-01)
T Fukamizo et al.
The Biochemical journal, 311 ( Pt 2), 377-383 (1995-10-15)
Chitosanase was produced by the strain of Streptomyces lividans TK24 bearing the csn gene from Streptomyces sp. N174, and purified by S-Sepharose and Bio-Gel A column chromatography. Partially (25-35%) N-acetylated chitosan was digested by the purified chitosanase, and structures of
Boucher, I., et al.
The Journal of Biological Chemistry, 270, 31-31 (1995)
Ryszard Brzezinski
Bioengineered bugs, 2(4), 226-229 (2011-08-11)
There is a growing interest in chitosanases as enzymatic tools to hydrolyze chitosan into bioactive forms: low molecular weight chitosan (LMWC) or chitosan oligosaccharides (CHOS). However chitosanases are still expensive and methods of large-scale production of these enzymes are not
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Articles

Enzymatic Assay of Chitosanase from Streptomyces Species

To standardize a procedure for determining the enzymatic activity of Chitosanase.

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