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BCK-HTS594-20

EdU HTS Kit 594

Name: EdU HTS Kit 594
Brand: SIGMA

sufficient for 2000 assays

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About This Item

Code UNSPSC :

12161503

Fluorescence

λ_ex 584 nm; λ_em 603 nm

Conditions d'expédition

wet ice

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Description générale

The Baseclick EdU HTS Kits provide a superior alternative to BrdU and [3H]thymidine assays for directly measuring DNA synthesis of adherent cells in 96 well plates. EdU (5-ethynyl-2′-deoxyuridine) is a nucleoside analog to thymidine and is incorporated into DNA during active DNA synthesis. In contrast to BrdU assays, the EdU HTS Assays are not antibody based and therefore do not require DNA denaturation for detection of the incorporated nucleoside. Instead, the EdU HTS Kits utilize click chemistry for detection in a variety of dye fluorescent readouts. Furthermore, the streamlined detection protocol reduces both the total number of steps and significantly decreases the total amount of time. The simple click chemistry detection procedure is complete within 30 minutes and is compatible with multiplexing for content and context-rich results.

Application

High Throughput Screening

Pictogrammes

GHS08,GHS05,GHS07

Mention d'avertissement

Danger

Mentions de danger

H302 + H332,H314,H340,H361,H412

Conseils de prudence

P273 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338

Classification des risques

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Muta. 1B - Repr. 2 - Skin Corr. 1C

Risques supp

EUH032

Code de la classe de stockage

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

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EdU incorporation is an alternative non-radioactive assay to [(3)H]thymidine uptake for in vitro measurement of mice T-cell proliferations.

Yongmao Yu et al.

Journal of immunological methods, 350(1-2), 29-35 (2009-08-04)

T lymphocyte proliferations can be measured by [(3)H]thymidine incorporation. However, many labs avoid this technique because of the need to use radioactive substrates. In addition, [(3)H]thymidine incorporation method does not permit simultaneous characterization of the proliferating cells. We developed the

EdU, a new thymidine analogue for labelling proliferating cells in the nervous system.

Fatemah Chehrehasa et al.

Journal of neuroscience methods, 177(1), 122-130 (2008-11-11)

Labelling and identifying proliferating cells is central to understanding neurogenesis and neural lineages in vivo and in vitro. We present here a novel thymidine analogue, ethynyl deoxyuridine (EdU) for labelling dividing cells, detected with a fluorescent azide which forms a

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