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B7906

Sigma-Aldrich

Butan-1-ol

for molecular biology, ≥99%

Synonyme(s) :

n-butanol, Alcool butylique

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About This Item

Formule linéaire :
CH3(CH2)3OH
Numéro CAS:
71-36-3
Poids moléculaire :
74.12
Numéro Beilstein :
969148
Numéro CE :
200-751-6
Numéro MDL:
MFCD00002964
Code UNSPSC :
12352200
ID de substance PubChem :
24892030
Nomenclature NACRES :
NA.52

Qualité

for molecular biology

Densité de vapeur

2.55 (vs air)

Pression de vapeur

5 (4 mmHg) at 20 °C hPa

Pureté

≥99%

Forme

liquid

Température d'inflammation spontanée

649 °F

Limite d'explosivité

11.2 %

Couleur

colorless

Indice de réfraction

n20/D 1.399 (lit.)

pH

7 (20 °C, 70 g/L)

Point d'ébullition

116-118 °C (lit.)

Pf

−90 °C (lit.)

Densité

0.81 g/mL at 25 °C (lit.)

Température de stockage

room temp

Chaîne SMILES 

CCCCO

InChI

1S/C4H10O/c1-2-3-4-5/h5H,2-4H2,1H3

Clé InChI

LRHPLDYGYMQRHN-UHFFFAOYSA-N

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Description générale

1-Butanol is a clear, colorless alcohol that can be used for the removal of ethidium bromide from DNA purified by CsCl gradient ultracentrifugation. It is also suitable for use in the concentration of dilute nucleic acid solutions by repeated extractions, thus improving recovery by ethanol precipitation.

Application

1-Butanol has been used:
  • in the derivatization process for measuring methylated arginine levels in blood samples
  • in liquid-chromatography mass spectrometry (LC-MS) analysis of lysine modifications by deacetylase CobB
  • in the extraction of plant material from Vigna unguiculata
  • to extract and purify amplified human DNA samples before further amplification by PCR followed by sequencing

1-Butanol is suitable for use in nucleic acid purification and concentration protocols.

Pictogrammes

FlameCorrosionExclamation mark
GHS02,GHS05,GHS07

Mention d'avertissement

Danger

Classification des risques

Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Skin Irrit. 2 - STOT SE 3

Organes cibles

Respiratory system

Code de la classe de stockage

3 - Flammable liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

95.0 °F - Pensky-Martens closed cup

Point d'éclair (°C)

35 °C - Pensky-Martens closed cup

Équipement de protection individuelle

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Certificats d'analyse (COA)

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Protocoles

Ethidium Bromide Removal

Ethidium Bromide Removal

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