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Key Documents

B7389

Sigma-Aldrich

Anti-Rabbit IgG (whole molecule)–Biotin antibody produced in goat

affinity isolated antibody, buffered aqueous solution

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About This Item

Numéro MDL:
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46

Source biologique

goat

Conjugué

biotin conjugate

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

secondary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Technique(s)

direct ELISA: 1:20,000

Température de stockage

2-8°C

Modification post-traductionnelle de la cible

unmodified

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Description générale

IgGs are glycoprotein antibodies that modulate several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins. Anti-Rabbit IgG (whole molecule)-Biotin antibody is specific for rabbit IgGs. IgG antibody plays a crucial role in humoral immune responses such as placental transport, complement activation, phagocytosis and cell surface-receptor binding.

Immunogène

Purified rabbit IgG

Application

Anti-Rabbit IgG (whole molecule)-Biotin antibody is suitable for use in western blot using extracellular matrix protein extracts from the bone marrow of mice. The product can also be used for direct ELISA (1:20,000).
Anti-rabbit IgG (whole molecule)–biotin antibody can be used in enfragment analysis to visualize native GFP protein in stem cell tumours. This antibody can also be used in western blotting and immunohistochemistry. Further this can be used as a secondary antibody in quantified immunocytochemistry experiments. Anti-rabbit IgG (whole molecule)–biotin antibody reacts specifically with all rabbit immunoglobulins.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


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N J Medill et al.
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Jennifer Stefani et al.
Frontiers in cellular neuroscience, 12, 134-134 (2018-06-06)
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Marina Konopleva et al.
Cancer research, 65(21), 9962-9970 (2005-11-04)
Conditionally active forms of the Raf proteins (Raf-1, B-Raf, and A-Raf) were created by ligating NH2-terminal truncated activated forms (Delta) to the estrogen receptor (ER) hormone-binding domain resulting in estradiol-regulated constructs (DeltaRaf:ER). These different Raf:ER oncoproteins were introduced into the
Jerónimo Auzmendi et al.
Pharmaceuticals (Basel, Switzerland), 11(1) (2018-02-22)
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