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A9941

Sigma-Aldrich

2,2′-azino-bis(acide 3-éthylbenzothiazoline-6-sulfonique) diammonium salt

chromogenic, tablet

Synonyme(s) :

2,2′-azino-bis(3-éthylbenzothiazoline-6-sulfonate) de diammonium, AzBTS-(NH4)2

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About This Item

Formule empirique (notation de Hill):
C18H24N6O6S4
Numéro CAS:
Poids moléculaire :
548.68
Numéro Beilstein :
7329461
Numéro CE :
Numéro MDL:
Code UNSPSC :
12352204
ID de substance PubChem :
Nomenclature NACRES :
NA.21

product name

2,2′-azino-bis(acide 3-éthylbenzothiazoline-6-sulfonique) diammonium salt, tablet, 10 mg substrate per tablet

Niveau de qualité

Forme

tablet

Solubilité

deionized water: 100 mL

Température de stockage

room temp

Chaîne SMILES 

[NH4+].[NH4+].CCN1C(\Sc2cc(ccc12)S([O-])(=O)=O)=N\N=C3/Sc4cc(ccc4N3CC)S([O-])(=O)=O

InChI

1S/C18H18N4O6S4.2H3N/c1-3-21-13-7-5-11(31(23,24)25)9-15(13)29-17(21)19-20-18-22(4-2)14-8-6-12(32(26,27)28)10-16(14)30-18;;/h5-10H,3-4H2,1-2H3,(H,23,24,25)(H,26,27,28);2*1H3/b19-17-,20-18-;;

Clé InChI

OHDRQQURAXLVGJ-AXMZSLBLSA-N

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Description générale

ABTS mainly used in ELISA (enzyme-linked immunosorbent assay) procedures.
ABTS, 2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid), is a chromogenic substrate used:
  • For a reaction with laccase or bilirubin oxidase
  • For a reaction with peroxidase enzymes: (like HRP for example). ABTS in combination with hydrogen peroxide
A peroxidase reaction of ABTS in the presence of hydrogen peroxide produces a green soluble end product which can be read spectrophotometrically at 405nm. The reaction may be stopped with 1% sodium dodecyl sulfate (SDS).
ABTS, 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt is light sensitive, and water soluble chemical compound

Application

  • 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, ABTS, has been used as a substrate in ELISA (enzyme-linked immunosorbent assay).†
  • ABTS is also used to measure glucose concentrations in different solutions including blood serum.
  • The food industry uses ABTS to measure the antioxidant capacities within different foods.
  • Since it is less readily oxidized than TMB and OPD substrates, ABTS is less sensitive for ELISA applications. Thus ABTS has some advantageous over TMB and OPD in cases of large background results
  • It is recommended for ELISA (microwell) procedures, not recommended for membrane applications.
L'acide 2,2′-azino-bis(3-éthylbenzothiazoline-6-sulfonique) est un substrat de la peroxydase qui peut être utilisé dans les protocoles ELISA. Il permet d'obtenir un produit final de couleur verte soluble, mesurable par spectrophotométrie à 405 nm. La réaction peut être arrêtée par l'ajout de dodécylsulfate de sodium (SDS) à 1 %. Ce substrat est recommandé pour les protocoles ELISA (micropuits) ; il n'est pas recommandé pour les techniques à membrane.

Reconstitution

Dissolve 1 tablet in 100 ml of 0.05 M phosphate-citrate buffer, pH 5.0 (Tablets available as Sigma Product No. P4809). Add 25 μl of fresh 30% hydrogen peroxide

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

dust mask type N95 (US), Eyeshields, Gloves


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

B Porstmann et al.
Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie, 19(7), 435-439 (1981-07-01)
o-Phenylenediamine, 2,2'-azino-di(3-ethylbenzthiazoline sulphonic acid-6) (ABTS), o-dianisidine and 4-aminoantipyrine were compared as chromogens for the determination of horseradish peroxidase. Highest sensitivity in the determination of horseradish peroxidase-IgG conjugates in dissolved form was obtained with o-phenylenediamine. When these conjugates were used in
Vignesh Narayanaswamy et al.
Breastfeeding medicine : the official journal of the Academy of Breastfeeding Medicine, 16(12), 987-994 (2021-08-13)
Objective: To evaluate the immune response to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in colostrum from women who tested positive for the virus. Methods: Between March and September 2020 we obtained bilateral colostrum samples collected on spot cards within
H Gallati
Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie, 17(1), 1-7 (1979-01-01)
The reaction of the two substrates hydrogen peroxide and ABTS with horseradish peroxidase was studied kinetically. Enzyme activity was determined as a function of substrate concentration and pH. Michaelis constants were determined for the two substrates at various pH values.
Da Di et al.
Pathogens (Basel, Switzerland), 10(8) (2021-08-29)
The SARS-CoV-2 nucleocapsid protein (N) binds a single-stranded viral RNA genome to form a helical ribonucleoprotein complex that is packaged into virion particles. N is relatively conserved among coronaviruses and consists of the N-terminal domain (NTD) and C-terminal domain (CTD)
Development of a feline proinsulin immunoradiometric assay and a feline proinsulin enzyme-linked immunosorbent assay (ELISA): A novel application to examine beta cell function in cats
Kley S, et al.
Domestic Animal Endocrinology, 311-318 (2008)

Articles

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

Protocoles

This procedure may be used for all Alcohol Oxidase products. The continuous spectrophotometric rate determination (A405, Light path = 1 cm) is based on the following reactions.

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