A6979

Anti-AtMPK4 antibody produced in rabbit

affinity isolated antibody, liquid

• Synonyme(s) : Anti-Arabidopsis thaliana MPK4
• Numéro MDL: MFCD07370260
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.44

Propriétés

• Source biologique: rabbit
• Conjugué: unconjugated
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Qualité: for molecular biology
• Clone: polyclonal
• Forme: liquid
• Poids mol.: antigen 43 kDa
• Espèces réactives: Arabidopsis thaliana
• Technique(s): western blot: 0.5-1 μg/mL using extract (cytosolic fraction) of Arabidopsis thaliana and Nicotiana tabacum leaves
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: Arabidopsis thaliana ... ATMPK4(828151)

Description

Description générale

Anti-AtMPK4 is produced in rabbit using as immunogen a synthetic peptide corresponding to the C-terminus of Arabidopsis thaliana MPK4 (Anti- mitogen-activated protein kinase-4), conjugated to KLH. In Arabidopsis thaliana, 20 genes (MPKs) encode putative plant MAPKs, which can be grouped into four or five classes according to sequence similarity. All plant mitogen-activated protein kinase (MAPKs) are classified into the plant extracellular-regulated kinase (PERK) subfamily.

Mitogen-activated protein kinase-4 (MPK4) is expressed in the nucleus. In pre-mitotic and interphase cells, it is found in the cytoplasm.

Immunogène

synthetic peptide corresponding to the C-terminus of Arabidopsis thaliana MPK4 (amino acids 365-376). This sequence is specific to AtMPK4 (not found in the other 20 known AtMPK isoforms). It shares limited homology (50-60% identity, continuous epitope) to the AtMPK5 and AtMPK12 isoforms.

Application

Anti- mitogen-activated protein kinase-4 (AtMPK4) antibody produced in rabbit has been used for Western blotting.

Anti-AtMPK4 antibody has been used in

• immunoblotting
• immunoprecipitation
• co-immunoprecipitation
• western blotting

Actions biochimiques/physiologiques

In Arabidopsis thaliana putative functions have been assigned to AtMPK3, AtMPK4, and AtMPK6, and all three mitogen-activated protein kinase (MAPKs) have been linked to diverse stress responses, including oxidative stress signaling, pathogen defense, drought, high salt, cold, and touch. Arabidopsis MAPKs, Anti- mitogen-activated protein kinase-3 (AtMPK3), Anti- mitogen-activated protein kinase-4 (AtMPK4), and Anti- mitogen-activated protein kinase-6 (AtMPK6) are rapidly and transiently activated by stress responses, by activation of distinct signal transduction pathways, involving upstream MAPKKKs (e.g. AtANP1 and AtMEKK1) and MAPKKs including AtMKK1/2 and AtMKK4/5.

Mitogen-activated protein kinase-4 (MPK4) negatively modulates systemic-acquired resistance. It functions as a regulator of retrograde signaling for photosynthesis, growth and immune defenses. It also has a role in cytokinesis and microtubule organization.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable