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Key Documents

A6792

Sigma-Aldrich

Anti-Dog IgG (whole molecule)−Peroxidase antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonyme(s) :

Rabbit Anti-Dog IgG (whole molecule)−HRP

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About This Item

Numéro MDL:
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46

Source biologique

rabbit

Niveau de qualité

Conjugué

peroxidase conjugate

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

secondary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Technique(s)

direct ELISA: 1:10,000

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

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Description générale

IgG (immunoglobulin G) antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids. Mammalian IgG has four subclasses- IgG1, IgG2a, IgG2b and IgG2c.
Specificity of the anti-dog IgG antiserum is determined by immunoelectrophoresis, prior to conjugation, versus normal dog serum and dog IgG.

Application

Anti-Dog IgG (whole molecule) Peroxidase antibody produced in rabbit has been used:
  • for the detection of antibodies against noro viruses, by ELISA (enzyme linked immunosorbent asay) at 1:2000 for 1 hour at 37°C
  • in IgG (Immunoglobulin G) staining method
  • in ELISA to detect the incorporation of RVGTM (a rabies virus G protein variant)

Actions biochimiques/physiologiques

IgG (Immunoglobulin G) antibody provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. Anti-Dog IgG antibody is a secondary antibody. Second antibodies (secondary antibodies) are raised against primary antibodies, which bind to specific antigens to create antigen antibody complexes.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin and 0.05% MIT

Notes préparatoires

Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in "Immunofluorescence and Related Staining Techniques," Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).

Informations légales

Ampliflu is a trademark of Sigma-Aldrich Co. LLC

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Pictogrammes

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Mention d'avertissement

Warning

Mentions de danger

Classification des risques

Skin Sens. 1

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

I F G de Amorim et al.
Veterinary parasitology, 173(1-2), 55-63 (2010-07-20)
Dogs represent the major reservoir of Leishmaniao chagasi and vaccination against the canine disease is a potential control strategy. However, seroconversion occurs post-vaccine and hence, there is need to discriminate between the former group and naturally infected dogs. The present
Maria M Figueiredo et al.
BMC immunology, 14, 22-22 (2013-05-15)
Infection with parasite protozoa is a long-term health issue in tropical and subtropical regions throughout the world. The Toll-like receptor (TLR) signaling pathway is one of the first-responding defense systems against Leishmania. The aim of this study was to investigate
Chimeric rabies glycoprotein with a transmembrane domain and cytoplasmic tail from Newcastle disease virus fusion protein incorporates into the Newcastle disease virion at reduced levels
Yu GM, et al.
Journal of Veterinary Science, 18(S1), 351-359 (2017)
Vivian Tamietti Martins et al.
PloS one, 10(9), e0137683-e0137683 (2015-09-15)
In the present study, two Leishmania infantum hypothetical proteins present in the amastigote stage, LiHyp1 and LiHyp6, were combined with a promastigote protein, IgE-dependent histamine-releasing factor (HRF); to compose a polyproteins vaccine to be evaluated against L. infantum infection. Also
Lukas Uhrik et al.
The Biochemical journal, 478(1), 99-120 (2020-12-08)
A comparative canine-human therapeutics model is being developed in B-cell lymphoma through the generation of a hybridoma cell that produces a murine monoclonal antibody specific for canine CD20. The hybridoma cell produces two light chains, light chain-3, and light chain-7.

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