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Key Documents

A6684

Sigma-Aldrich

Monoclonal Anti-Albumin antibody produced in mouse

clone HSA-11, ascites fluid

Synonyme(s) :

Anti-AFP

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About This Item

Numéro MDL:
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46

Source biologique

mouse

Niveau de qualité

Conjugué

unconjugated

Forme d'anticorps

ascites fluid

Type de produit anticorps

primary antibodies

Clone

HSA-11, monoclonal

Contient

15 mM sodium azide

Espèces réactives

gibbon, baboon, human, rhesus monkey

Ne doit pas réagir avec

turkey, feline, hamster, pigeon, rat, marmoset, pig, bovine, canine, donkey, guinea pig, rabbit, chicken, sheep, goat

Technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
indirect ELISA: 1:10,000
western blot: 1:5,000

Isotype

IgG2a

Numéro d'accès UniProt

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... ALB(213)

Description générale

Monoclonal Anti-Albumin (mouse IgG2a isotype) is derived from the HSA-11 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Albumin, the major protein produced by the liver, represents more than half the total protein in human serum. Many other body fluids also contain albumin.
Serum albumin is the major protein component of blood and is produced in the liver. It regulates colloid osmotic blood pressure, transportation of a variety of substances, blood pH and provides an endogenous source of amino acids. Monoclonal anti-albumin antibody (diluted 1:100) can be used in double staining to localize hepatocytes differentiated from human umbilical mesenchymal stem cells (HUMSCs) . Offering specificity for human serum albumin, this product is ideal for identification of human blood stains or other body fluids (e.g., seminal fluid, milk, urine, saliva, vaginal secretions). Mouse monoclonal anti-albumin antibody reacts specifically with denatured and reduced human serum albumin and cross reacts with rhesus, gibbon and baboon serum albumins. The product has shown no cross reactivity with bovine, cat, chicken, dog, donkey, goat, guinea pig, hamster, marmoset, pig, pigeon, rabbit, rat, sheep, or turkey serum albumin or with chicken and turkey egg albumin.

Spécificité

The antibody is specific for human albumin in serum and plasma and recognizes an epitope resistant to reduction or denaturation.

Immunogène

Human serum albumin

Application

Monoclonal Anti-Albumin antibody produced in mouse has been used in:
  • immunofluorescence
  • staining
  • double staining
  • enzyme-linked immunosorbent assay (ELISA)
  • immunoblot
  • immunohistochemistry

Actions biochimiques/physiologiques

Albumin is a transport protein that binds a broad range of ligands such as fatty acids, bilirubin, hemin, drugs, amino acids and ions . Albumin may also function as a zinc carrier protein and consequently regulate physiological processes .
High levels of albumin are associated with dehydration. Albumin turnover is seen in infants with iron deficiency anemia. Oxidative damage of albumin is associated with advanced liver disease.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

13 - Non Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

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Clinical use of albumin
Caraceni P, et al.
Blood Transfusion = Trasfusione del Sangue, 11(Suppl 4), s18-s18 (2013)
Katy M Olsavsky et al.
Toxicology and applied pharmacology, 222(1), 42-56 (2007-05-22)
Frequently, primary hepatocytes are used as an in vitro model for the liver in vivo. However, the culture conditions reported vary considerably, with associated variability in performance. In this study, we characterized the differentiation character of primary human hepatocytes cultured
Efficient generation of functional hepatocyte-like cells from human fetal hepatic progenitor cells in vitro
Zhang W, et al.
Journal of Cellular Physiology, 227(5), 2051-2058 (2012)
Yoon Ok Jang et al.
BMC gastroenterology, 14, 198-198 (2014-11-27)
Cirrhosis is a long-term consequence of chronic hepatic injury with fibrosis. No effective therapy is currently available for decompensated cirrhosis except liver transplantation. Hence, we investigated the effect of bone marrow-derived mesenchymal stem cells (BM-MSCs) on hepatic fibrosis in a
Obinna C Ubah et al.
Frontiers in immunology, 8, 1780-1780 (2018-01-10)
The management of chronic inflammatory diseases, such as inflammatory bowel disease, psoriasis, and rheumatoid arthritis has significantly improved over the last decade with the clinical availability of anti-TNF-α biologics. Despite this undoubted treatment success, a combination of acquired resistance together

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