A5420
Anti-Goat IgG (whole molecule)–Peroxidase antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
Synonyme(s) :
Anti Goat Igg, Anti-Goat IgG (whole molecule)–Peroxidase antibody produced in rabbit
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About This Item
Produits recommandés
Source biologique
rabbit
Niveau de qualité
Conjugué
peroxidase conjugate
Forme d'anticorps
affinity isolated antibody
Type de produit anticorps
secondary antibodies
Clone
polyclonal
Forme
buffered aqueous solution
Espèces réactives
goat
Technique(s)
direct ELISA: 1:40,000
dot blot: 1:80,000-1:160,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200
Conditions d'expédition
dry ice
Température de stockage
−20°C
Modification post-traductionnelle de la cible
unmodified
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Catégories apparentées
Description générale
Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chain and two light chain connected by disulfide bond. It is a glycoprotein and a major class of immunoglobulin. Goat IgG has two subclasses- IgG1 and IgG2.
Goat IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-goat IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins. Rabbit anti-goat IgG (whole molecule)-peroxidase antibody binds to all goat Igs.
Goat IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-goat IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins. Rabbit anti-goat IgG (whole molecule)-peroxidase antibody binds to all goat Igs.
Spécificité
Specificity of Anti-Goat IgG- Peroxidase is determined by immunoelectrophoresis (IEP) versus normal goat serum and goat IgG.
Immunogène
Purified goat IgG
Application
LNCAP cell lysates were analyzed by western blot using HRP conjugated rabbit anti-goat IgG as the secondary at a 1:7500 dilution in TBSt for 1 hour at room temperature.
Rabbit Anti-Goat IgG (whole molecule)-Peroxidase antibody has been used for immunoblotting assays. The antibody can also be used for direct ELISA (1:40,000), dot blot (1:80,000-1:160,000) and immunohistochemistry (1:200) assays.
Anti-Goat IgG (whole molecule)–Peroxidase antibody has been used in
Anti-Goat IgG (whole molecule)–Peroxidase antibody has been used in
- western blotting
- immunohistochemical and immunofluorescent analysis
- visualization of cross-reactivity
- protein analysis
- indirect ELISA for detection of specific IgG against C. pseudotuberculosis
- immunoprecipitation (IP)
Actions biochimiques/physiologiques
Immunoglobulin G (IgG) glycoprotein antibodies modulates several immune responses. It mainly participates in hypersensitivity type II and type III.
Forme physique
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT
Stockage et stabilité
For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Skin Sens. 1
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Evaluation of the humoral and cellular immune response to different antigens of Corynebacterium pseudotuberculosis in Caninde goats and their potential protection against caseous lymphadenitis.
Moura-Costa L F, et al.
Veterinary Immunology and Immunopathology, 126(1-2), 131-141 (2008)
Adenosine augments IL-10 production by macrophages through an A2B receptor-mediated posttranscriptional mechanism.
Nemeth Z. H, et al.
Journal of Immunology, 175(12), 8260-8270 (2005)
Y Yu et al.
British journal of pharmacology, 165(1), 148-166 (2011-06-11)
Our previous studies demonstrated that a thiosemicarbazone iron chelator (di-2-pyridylketone-4,4-dimethyl-3-thiosemicarbazone; Dp44mT) possesses potent and selective anti-cancer activity but led to cardiotoxicity at non-optimal doses. In this study, we examined the in vivo anti-tumour efficacy and tolerability of a new-generation 2-benzoylpyridine
Respiratory syncytial virus nonstructural proteins decrease levels of multiple members of the cellular interferon pathways.
Swedan S, et al.
Journal of Virology, 83(19), 9682-9693 (2009)
Properdin plays a protective role in polymicrobial septic peritonitis.
Stover C M, et al.
Journal of Immunology, 180(5), 3313-3318 (2008)
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