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A5236

Sigma-Aldrich

Anti-ASF1 antibody, Mouse monoclonal

clone 4A1/3, purified from hybridoma cell culture

Synonyme(s) :

Anti-ASF1A, Anti-Silencing function 1 homolog A

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About This Item

Code UNSPSC :
12352203

Source biologique

mouse

Niveau de qualité

200

Conjugué

unconjugated

Forme d'anticorps

purified from hybridoma cell culture

Type de produit anticorps

primary antibodies

Clone

4A1/3, monoclonal

Forme

buffered aqueous solution

Poids mol.

antigen ~23 kDa

Espèces réactives

mouse, human

Concentration

~1.0 mg/mL

Technique(s)

western blot: 2-4 μg/mL using whole extract of human Jurkat or Raji cells

Isotype

IgG2a

Numéro d'accès UniProt

Q9Y294

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... ASF1A(25842)

Description générale

ASF1 is a histone chaperone protein that binds H3 and H4. It functions in nucleosome assembly.
Monoclonal Anti-ASF1 (mouse IgG2a isotype) is derived from the hybridoma 4A1/3 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with an ASF1A fusion protein. ASF1 (anti-silencing function 1) encodes a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast.

Immunogène

ASF1A fusion protein

Application

Monoclonal Anti-ASF1 antibody produced in mouse has been used in immunoblotting.

Actions biochimiques/physiologiques

ASF1 (anti-silencing function 1) protein is a key component of a histone donor complex. It interacts with histones H3 and H4 and functions together with a chromatin assembly factor 1 (CAF-1) during DNA replication and repair. ASF1 also contributes to genome stability during S phase. Cells lacking ASF1 were found to be sensitive to a number of genotoxic agents that predominantly cause DNA damage by interfering with DNA replication fork progression such as hydroxyurea and camptothecin.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

ANTI-SILENCING FUNCTION1 proteins are involved in ultraviolet-induced DNA damage repair and are cell cycle regulated by E2F transcription factors in Arabidopsis
Lario LD, et al.
Plant Physiology, 162(2), 1164-1177 (2013)
Histone deposition protein Asf1 maintains DNA replisome integrity and interacts with replication factor C
Franco AA, et al.
Genes & Development, 19(11), 1365-1375 (2005)
Differential requirements for Tousled-like kinases 1 and 2 in mammalian development
Segura-BS, et al.
Cell Death and Differentiation, 24(11), 1872-1872 (2017)
Sandra Segura-Bayona et al.
Cell death and differentiation, 24(11), 1872-1885 (2017-07-15)
The regulation of chromatin structure is critical for a wide range of essential cellular processes. The Tousled-like kinases, TLK1 and TLK2, regulate ASF1, a histone H3/H4 chaperone, and likely other substrates, and their activity has been implicated in transcription, DNA

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