A4327

Albumine from human serum

lyophilized powder, essentially protease free, ≥96% (agarose gel electrophoresis)

• Synonyme(s) : HSA
• Numéro CAS: 70024-90-7
• Numéro CE : 274-272-6
• Numéro MDL: MFCD00081418
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.25

Propriétés

• Source biologique: human
• Niveau de qualité: 200
• Pureté: ≥96% (agarose gel electrophoresis)
• Forme: lyophilized powder
• Poids mol.: monomer calculated mol wt 66478 Da
• Technique(s): tissue culture: suitable
• Impuretés: HIV I and HIVII, HCV and HBsAg, tested negative
• Solubilité: H₂O: soluble 50 mg/mL
• Numéro d'accès UniProt: P02768
• Activité étrangère: Alkaline phosphatase ≤0.001 units/mg protein; Peroxidase ≤0.001 units/mg protein; Protease ≤0.0001 units/mg protein
• Température de stockage: 2-8°C
• InChI: 1S/C3F8/c4-1(5,2(6,7)8)3(9,10)11
• Clé InChI: QYSGYZVSCZSLHT-UHFFFAOYSA-N
• Informations sur le gène: human ... ALB(213)

Description

Description générale

Albumin is the most copious protein in blood plasma. Produced in the liver, serum albumin serves as a carrier protein for biomolecules like as fatty acids, amino acids, and steroids.

This human serum albumin (HSA) product has been specially tested and evaluated to be low in the following properties:

• Essentially protease-free / low protease content
• Low alkaline phosphatase content
• Low peroxidase content

Serum albumin is used in various applications related to molecular biology, notably as an inert protein stabilizer for stock solutions of enzymes and proteins of interest. In such applications, it is thus important for the HSA to be protease free, to maintain the integrity of the protein or enzyme of interest.

Protease-free HSA is especially useful for applications like:

• Enzyme assays
• Protein-based assays
• Protease-sensitive techniques such as:

(a) Enzyme immunoassay (EIA)
(b) Nucleic acid hybridization
(c) Radioimmunoassay (RIA)

Application

Albumin was used to test its effect on the in vitro bactericidal activity of cefditoren against penicillin-resistant Streptococcus pneumonia. It has been clinically used in serious and often life-threatening conditions, such as shock and blood loss due to trauma, burns, and surgery.
It was used to test the effect of non-enzymatic glycation on the unfolding of human serum albumin. It was also used to test the two distinct pathways used by intestinal epithelial cells for HLA class II antigen processing.

Actions biochimiques/physiologiques

L′albumine sérique fonctionne en tant que protéine porteuse des stéroïdes, acides gras et hormones thyroïdes, et est essentielle à la régulation de la pression colloïde osmotique du sang. L′albumine est également capable de se lier à certaines substances exogènes, en particulier les médicaments (p. ex., ibuprofène, warfarine) et influence fortement leurs propriétés pharmacocinétiques. Le stress oxydant, qui entraîne des changements de l′état d′oxydoréduction de l′albumine, exerce des effets divers sur sa fonction physiologique.

Caractéristiques et avantages

• Low protease activity / essentially protease-free (≤ 0.0001 units/mg protein)
• Low alkaline phosphatase activity (≤ 0.001 units/mg protein)
• Low peroxidase activity (≤ 0.001 units/mg protein)

Notes préparatoires

Prepared from A1653

Autres remarques

Obtenir de plus amples informations sur l′albumine sérique humaine.

Clause de non-responsabilité

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves