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A3547

Sigma-Aldrich

Achromopeptidase from bacteria

lyophilized powder, ≥1,000 units/mg solid

Synonyme(s) :

ACP, Lysyl endopeptidase

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About This Item

Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54

Source biologique

bacterial (LYSOBACTER SP. BACTERIA M497-1)

Niveau de qualité

200

Forme

lyophilized powder

Activité spécifique

≥1,000 units/mg solid

Poids mol.

27 kDa

Technique(s)

cell based assay: suitable

Adéquation

suitable for cell lysis

Application(s)

diagnostic assay manufacturing

Conditions d'expédition

dry ice

Température de stockage

−20°C

Application

Achromopeptidase is useful for lysis of Gram-positive bacteria that are resistant to lysozyme. Achromopeptidase, from Sigma, has been used for the isolation and molecular characterization of skin and soft-tissue bacterial infections.

Actions biochimiques/physiologiques

Achromopeptidase is a lysyl endopeptidase that has a molecular weight of approximately 27 kDa. It works best between pH 8.5 -9. Approximately 500-1,500 μn/ml achromopetidase can be used to lyse cells at a density of OD600 = 0.6 over 2 hours at 37 °C.
Achromopeptidase is a lysyl endopeptidase with a MW of ~27 kDa. It is useful for lysis of Gram-positive bacteria that are resistant to lysozyme.
pH Optimum for activity: pH 8.5 - 9
Approximately 500-1,500 un/ml achromopetidase can be used to lyse cells at a density of OD600=0.6 over 2 hours at 37 °C.

Définition de l'unité

One unit will lyse 0.4 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 8.0 at 37 °C.

Forme physique

crude powder

Pictogrammes

Health hazard
GHS08

Mention d'avertissement

Danger

Mentions de danger

H334

Conseils de prudence

P261 - P342 + P311

Classification des risques

Resp. Sens. 1

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Équipement de protection individuelle

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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Georgios Chondrogiannis et al.
Scientific reports, 11(1), 6140-6140 (2021-03-19)
Enzymes are the cornerstone of modern biotechnology. Achromopeptidase (ACP) is a well-known enzyme that hydrolyzes a number of proteins, notably proteins on the surface of Gram-positive bacteria. It is therefore used for sample preparation in nucleic acid tests. However, ACP
Yuanyuan Hu et al.
World journal of microbiology & biotechnology, 32(1), 1-1 (2015-11-26)
Extracting DNA from Staphylococcus aureus cells is important for detecting MRSA by PCR. However, S. aureus cells are known to be difficult to disrupt due to their compact cell walls. Here, we systematically studied the efficiency of a highly active
Chelsey A Smith et al.
Lab on a chip, 23(3), 451-465 (2022-12-24)
Cervical cancer is a leading cause of cancer death for women in low-resource settings. The World Health Organization recommends that cervical cancer screening programs incorporate HPV DNA testing, but available tests are expensive, require laboratory infrastructure, and cannot be performed
Adebayo Shittu et al.
Journal of medical microbiology, 53(Pt 1), 51-55 (2003-12-10)
The isolation, molecular identification and genotyping of multiresistant Staphylococcus sciuri and Staphylococcus haemolyticus from skin and soft-tissue infections are reported. Accurate and full identification of three coagulase-negative staphylococcal isolates was achieved using PCR, while the API STAPH method failed to

Protocoles

Enzymatic Assay of Achromopeptidase

Enzymatic Assay of Achromopeptidase

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