A2074
Monoclonal Anti-Rabbit Immunoglobulins–Peroxidase antibody produced in mouse
clone RG-16, purified immunoglobulin, lyophilized powder
Synonyme(s) :
Monoclonal Anti-Rabbit Immunoglobulins (IgG, IgA, IgM)
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About This Item
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46
Produits recommandés
Source biologique
mouse
Niveau de qualité
Conjugué
peroxidase conjugate
Forme d'anticorps
purified immunoglobulin
Type de produit anticorps
secondary antibodies
Clone
RG-16, monoclonal
Forme
lyophilized powder
Espèces réactives
rabbit
Ne doit pas réagir avec
pig, chicken, bovine, horse, human, guinea pig, turkey, rat, canine, goat, feline, sheep
Conditionnement
vial of 0.5 mL
Technique(s)
direct ELISA: 1:30,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using human tissue
western blot (chemiluminescent): 1:160,000
Isotype
IgG1
Température de stockage
2-8°C
Modification post-traductionnelle de la cible
unmodified
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Description générale
Monoclonal Anti-Rabbit Immunoglobulins (mouse IgG1 isotype) is derived from the RG-16 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with rabbit IgG. Immunoglobulins (Igs) belongs to the immunoglobulin super-family. There are five different types of Immunoglobulins. Each immunoglobulin has two heavy (H) and two light (L) chains held together by disulfide linkages. Each light comprises one variable N-terminal region and a constant C-terminal region. Heavy chain has one variable N-terminal region and three or four constant (CH1-CH4) C-terminal region. IgG is abundant immunoglobulin in human serum. The four classes of IgG can be IgG1, IgG2, IgG3, and IgG4.
Reacts with an epitope on the heavy chain of rabbit IgG, IgA and IgM, which is sensitive to reduction. No cross-reactivity with human IgG, IgA and IgM.
Spécificité
Binds only rabbit Igs. Does not react with reduced rabbit Igs.
Application
Monoclonal Anti-Rabbit Immunoglobulins-Peroxidase antibody produced in mouse has been used in:
- western blotting
- immunoblotting
- immunoprecipitation
- enzyme linked immunosorbent assay (ELISA)
- immunohistochemistry
Mouse monoclonal anti-rabbit immunoglobulins have been used for western blot assays at a dilution of 1:1000 using bovine trabecular meshwork cell lysates. The antibody can also be used for immunohistochemistry assays (1:100, using human tissue).
The mouse monoclonal anti-Rabbit IgG (heavy chain-specific) peroxidase conjugated antibody can be used as a secondary antibody for western blot analysis of protein preps from E. coli cultures at a concentration of 1:10000 diluted in PBSt for 1 hour at room temperature.
Forme physique
Lyophilized from 0.01 M sodium phosphate buffer, pH 7.4, containing 1% bovine serum albumin and 0.05% MIT
Notes préparatoires
Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Skin Sens. 1
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 2
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
Certificats d'analyse (COA)
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