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94046

Sigma-Aldrich

Phosphate de sodium dibasique solution

BioUltra, 0.5 M in H2O

Synonyme(s) :

Hydrogénophosphate disodique solution, Solution tampon d’hydrogénophosphate disodique

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About This Item

Formule linéaire :
Na2HPO4
Numéro CAS:
7558-79-4
Poids moléculaire :
141.96
Numéro MDL:
MFCD00003496
Code UNSPSC :
12161700
eCl@ss :
38070211
ID de substance PubChem :
57653505
Nomenclature NACRES :
NA.25

Gamme de produits

BioUltra

Niveau de qualité

100

Forme

liquid

Concentration

0.5 M in H2O

Impuretés

insoluble matter, passes filter test
≤0.001% total nitrogen (N)

pH

8.8-9.4 (25 °C, 0.5 M in H2O)

Solubilité

H2O: 0.5 M at 20 °C, clear, colorless

Densité

1.064 g/mL at 20 °C

Traces d'anions

chloride (Cl-): ≤10 mg/kg
sulfate (SO42-): ≤50 mg/kg

Traces de cations

Al: ≤1 mg/kg
As: ≤0.1 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
K: ≤20 mg/kg
Li: ≤1 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
Ni: ≤1 mg/kg
Sr: ≤1 mg/kg
Zn: ≤1 mg/kg

λ

0.5 M in H2O

Absorption UV

λ: 260 nm Amax: 0.019
λ: 280 nm Amax: 0.016

Application(s)

diagnostic assay manufacturing

Chaîne SMILES 

[Na+].[Na+].OP([O-])([O-])=O

InChI

1S/2Na.H3O4P/c;;1-5(2,3)4/h;;(H3,1,2,3,4)/q2*+1;/p-2

Clé InChI

BNIILDVGGAEEIG-UHFFFAOYSA-L

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Application

Sodium phosphate dibasic solution has been used:
  • to supplement Dulbecco′s Modified Eagle Medium (DMEM) in calcifying medium to induce the calcification of vascular smooth muscle cells (VSMCs)
  • to prepare a stock solution of CaCl2 and MgCl2 to synthesize calcium phosphate (CPB) and magnesium phosphate bions (MPBs) respectively
  • to supplement 2xYT agar plates to culture BL21 DE3 star cells and BL21dLac Gold cells

Sodium phosphate dibasic solution may be used in the preparation of a wide variety of phosphate based biological buffers for use in cell and molecular biology application.

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Daria Shishkova et al.
International journal of molecular sciences, 21(22) (2020-11-26)
Calciprotein particles (CPPs), which increasingly arise in the circulation during the disorders of mineral homeostasis, represent a double-edged sword protecting the human organism from extraskeletal calcification but potentially causing endothelial dysfunction. Existing models, however, failed to demonstrate the detrimental action
Megan A Rees et al.
Journal of proteome research, 14(1), 120-132 (2014-10-21)
Interactions between a host and a bacterial pathogen are mediated by cross-talk between molecules present on, or secreted by, pathogens and host binding-molecules. Identifying proteins involved at this interface would provide substantial insights into this interaction. Although numerous studies have
Natalie K Binder et al.
Molecular human reproduction, 21(5), 424-434 (2015-03-04)
The rate of obesity among men of reproductive age has tripled in the last three decades. Previously, we demonstrated that paternal obesity resulted in impaired preimplantation developmental kinetics, compromised post-compaction metabolism and decreased blastocyst cell number when embryos were generated
Johanna Troberg et al.
Drug metabolism and disposition: the biological fate of chemicals, 43(1), 107-118 (2014-10-11)
Understanding drug glucuronidation in the dog, a preclinical animal, is important but currently poorly characterized at the level of individual enzymes. We have constructed cDNAs for the 10 dog UDP-glucuronosyltransferases of subfamily 1A (dUGT1As), expressed them in insect cells, and
Jiwei Cui et al.
ACS nano, 9(2), 1571-1580 (2015-02-26)
We report the engineering of poly(ethylene glycol) (PEG) hydrogel particles using a mesoporous silica (MS) templating method via tuning the PEG molecular weight, particle size, and the presence or absence of the template and investigate the cell association and biodistribution
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Protocoles

Enzymatic Assay of Hyaluronidase (3.2.1.35)

This procedure may be used for Hyaluronidase products.

Enzymatic Assay of Alcohol Dehydrogenase (EC 1.1.1.1)

To measure alcohol dehydrogenase activity, this assay uses β-nicotinamide adenine dinucleotide phosphate and a continuous spectrophotometric rate determination at 340 nm.

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