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Supelco

Atto 590 NHS ester

suitable for fluorescence, BioReagent, ≥90.0% (degree of coupling)

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About This Item

Formule empirique (notation de Hill):
C41H42ClN3O11
Numéro CAS:
670269-33-7
Poids moléculaire :
788.24
Numéro MDL:
MFCD03456133
Code UNSPSC :
12352108
Nomenclature NACRES :
NA.32

Gamme de produits

BioReagent

Pureté

≥90.0% (HPLC)
≥90.0% (degree of coupling)

Forme

powder

Fabricant/nom de marque

ATTO-TEC GmbH

λ

in ethanol (with 0,1% trifluoroacetic acid)

Absorption UV

λ: 597-663 nm Amax

Adéquation

suitable for fluorescence

Méthode de détection

fluorometric

Température de stockage

−20°C

InChI

1S/C41H41N3O7.ClHO4/c1-9-42-31-18-33-29(16-26(31)22(3)20-40(42,5)6)37(30-17-27-23(4)21-41(7,8)43(10-2)32(27)19-34(30)50-33)28-15-24(11-12-25(28)38(47)48)39(49)51-44-35(45)13-14-36(44)46;2-1(3,4)5/h11-12,15-21H,9-10,13-14H2,1-8H3;(H,2,3,4,5)

Clé InChI

SHJDVERDESTYRQ-UHFFFAOYSA-N

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Description générale

Atto 590 is a novel fluorescent label belonging to the class of Rhodamine dyes, which has an absorption maximum of 594 nm and an emission maximum of 624 nm. Atto 590-N-hydroxysuccinimide (NHS) is membrane permeable.
Atto 590 is a novel fluorescent label belonging to the class of Rhodamine dyes. The dye is designed for application in the area of life science, e.g. labeling of DNA, RNA or proteins. The Atto dyes are a series of fluorescent dyes that provide all the crucial properties required for modern fluorescent technologies, such as fluorescence microscopy, flow-cytometry, fluorescence in situ hybridization (FISH), receptor binding assays or enzyme assays. The dye is highly suitable for single-molecule detection applications and high-resolution microscopy. The NHS-esters are used in common conjugation protocols.

Application

Atto 590 NHS ester may be suitable for use in site-specific labeling of human embryonic kidney (HEK293T) cell lysates for western blotting, fluorescence, and widefield microscopy studies.
Molar absorption 120,000 1/M cm, abs: 593 nm, em: 620 nm, QY=0.93, Tfl 4.0 ns (unpublished data)

Caractéristiques et avantages

  • Absorption maxima cover a range from 590 to 620 nm.
  • Structural properties support extraordinary photostability.
  • High fluorescence quantum yield and high thermal stability.
  • The fluorescence characteristics of the dye are quite insensitive to environmental changes (e.g., pH changes). This holds for both excitation and emission wavelengths and for the decay time of their fluorescence emission.
  • Compatible with common labeling procedures for proteins and amino-functionalized oligonucleotides.

Autres remarques

Study of multistep energy transfer in a single photonic wire by FRET; employed in fluorescence energy transfer, FRET, scanning microscopy.

Informations légales

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Y. Eberstein et al.
J. Chem. Phys., B 108, 93-93 (2004)
D Wildanger et al.
Journal of microscopy, 236(1), 35-43 (2009-09-24)
The advent of supercontinuum laser sources has enabled the implementation of compact and tunable stimulated emission depletion fluorescence microscopes for imaging far below the diffraction barrier. Here we report on an enhanced version of this approach displaying an all-physics based
Mike Heilemann et al.
Journal of the American Chemical Society, 126(21), 6514-6515 (2004-05-27)
We demonstrate the synthesis and spectroscopic characterization of an unidirectional photonic wire based on four highly efficient fluorescence energy-transfer steps (FRET) between five spectrally different chromophores covalently attached to double-stranded DNA. The DNA-based modular conception enables the introduction of various
Martin Baumdick et al.
Nature communications, 9(1), 3847-3847 (2018-09-23)
Epidermal growth factor receptor (EGFR) activation by growth factors (GFs) relies on dimerization and allosteric activation of its intrinsic kinase activity, resulting in trans-phosphorylation of tyrosines on its C-terminal tail. While structural and biochemical studies identified this EGF-induced allosteric activation
Uffe V Schneider et al.
Nucleic acids research, 38(13), 4394-4403 (2010-03-27)
Twisted intercalating nucleic acid (TINA) is a novel intercalator and stabilizer of Hoogsteen type parallel triplex formations (PT). Specific design rules for position of TINA in triplex forming oligonucleotides (TFOs) have not previously been presented. We describe a complete collection
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