78363
Atto 490LS maleimide
suitable for fluorescence
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About This Item
Code UNSPSC :
12352111
Nomenclature NACRES :
NA.32
Produits recommandés
Essai
≥90% (HPLC)
Niveau de qualité
Forme
(Report result)
Poids mol.
Mw 818 g/mol
Fabricant/nom de marque
ATTO-TEC GmbH
Transmittance
254 nm, >90%
490 nm, >90%
Fluorescence
λex 496 nm; λem 640.0 nm±10 nm in PBS, pH 7.4
λ
in PBS, pH 7.4
Absorption UV
λ: 494-500 nm Amax
Adéquation
suitable for fluorescence
Température de stockage
−20°C
Description générale
Atto 490LS is a new fluorescent label featuring an extraordinary large Stokes-Shift of 165 nm. Thus the emission spectrum is almost completely separated from its absorption spectrum, making the dye highly suitable for multiplexing experiments, in particular in combination with Atto 488 and Atto 514. Atto 490LS is very hydrophillic and shows excellent water solubility. The dye exhibits a relatively high fluorescence quantum yield, which is only slightly reduced after conjugation to biomolecules, e.g. proteins, even at high degrees of labeling (DOL). Atto 490LS is an anionic dye. After conjugation to a substrate the dye carries a net electrical charge of -1.
Maleimides are well suited for coupling to thiol groups. This is similar to iodacetamides, but maleimides do react more thiol selective. They do not show significant reaction with histidine or methionine. Hydrolysis of maleimides to a mixture of isomeric nonreactive maleamic acids can compete significantly with thiol modification, particularly above pH 8. Maleimides may be used for labelling of amines, which usually requires a higher pH than reaction of maleimides with thiols.
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Maleimides are well suited for coupling to thiol groups. This is similar to iodacetamides, but maleimides do react more thiol selective. They do not show significant reaction with histidine or methionine. Hydrolysis of maleimides to a mixture of isomeric nonreactive maleamic acids can compete significantly with thiol modification, particularly above pH 8. Maleimides may be used for labelling of amines, which usually requires a higher pH than reaction of maleimides with thiols.
find more information here
Informations légales
This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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