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57620

Sigma-Aldrich

Inulinase from Aspergillus niger

lyophilized, powder, brown-gray, ~25 U/mg

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About This Item

Numéro CAS:
Numéro de classification (Commission des enzymes):
Numéro CE :
Numéro MDL:
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54

Source biologique

Aspergillus niger

Forme

powder

Qualité

lyophilized

Activité spécifique

~25 U/mg

Couleur

brown-gray

Température de stockage

2-8°C

Description générale

Inulinases are classified as exoinulinases and endoinulinases.

Application

Inulinase from Aspergillus niger has been used as a glycoside hydrolase (GH) to study its potential to disperse bacteria (P. aeruginosa and S. aureus) from microbial biofilms using a polymicrobial well-plate model.

Actions biochimiques/physiologiques

Inulinase is used in high-fructose syrup, which has therapeutic applications. Inulinase is reported to have high activity toward sucrose.
Inulinase hydrolyses inulin to produce oligosaccharides and liberate fructose. It also splits the terminal fructose units into sucrose and raffinose.

Définition de l'unité

1 U corresponds to the amount of enzyme which releases 1 μmol of reducing sugar (measured as fructose) per minute at pH 4.1 and 37°C from inulin

Autres remarques

Characterization

Pictogrammes

Health hazard

Mention d'avertissement

Danger

Mentions de danger

Conseils de prudence

Classification des risques

Resp. Sens. 1

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)


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Consulter la Bibliothèque de documents

R. Azhari et al.
Biotechnology and Applied Biochemistry, 11, 105-105 (1989)
Gonçalo L M Santa et al.
Applied biochemistry and biotechnology, 165(1), 1-12 (2011-03-30)
The present work aims to provide the basic characterization of sol-gel immobilized inulinase, a biocatalyst configuration yet unexploited, using as model system the hydrolysis of inulin to fructose. Porous xerogel particles with dimensions in slight excess of 10 μm were obtained
Pierre Delanaye et al.
Clinica chimica acta; international journal of clinical chemistry, 413(5-6), 556-560 (2011-12-14)
Among issues susceptible to hamper a reliable measurement of inulin clearance, those regarding the dosage of inulin are largely neglected. We have compared the analytical performances of 2 commonly used methods of inulin dosage (one "acid" and one "enzymatic" method)
Wen Wan et al.
Applied microbiology and biotechnology, 91(3), 789-798 (2011-06-10)
Easy and low-cost protein purification methods for the mass production of commonly used enzymes that play important roles in biotechnology are in high demand. In this study, we developed a fast, low-cost recombinant protein purification system in the methylotrophic yeast
Takahiro Rikita et al.
Analytical biochemistry, 426(1), 24-26 (2012-04-10)
Inulin is a polysaccharide composed mainly of D-fructose units and is the most reliable indicator of the glomerular filtration rate. We have proposed an inulin detection method that involves the hydrolysis of inulin to D-fructose using inulinase and the selective

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