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53048

Sigma-Aldrich

5-Carboxy-tetramethylrhodamine N-succinimidyl ester

BioReagent, suitable for fluorescence

Synonyme(s) :

"4-Carboxytetramethylrhodamine N-succinimidyl ester", 5-TAMRA

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About This Item

Formule empirique (notation de Hill):
C29H25N3O7
Numéro CAS:
Poids moléculaire :
527.52
Numéro MDL:
Code UNSPSC :
12352116
ID de substance PubChem :
Nomenclature NACRES :
NA.32

Gamme de produits

BioReagent

Niveau de qualité

Solubilité

DMF: soluble
acetonitrile: soluble

Fluorescence

λex 543 nm; λem 578 nm in 0.1 M phosphate pH 7.0

Adéquation

suitable for fluorescence

Température de stockage

−20°C

Chaîne SMILES 

CN(C)c1ccc2c(OC3=C\C(C=CC3=C2c4ccc(cc4C([O-])=O)C(=O)ON5C(=O)CCC5=O)=[N+](\C)C)c1

InChI

1S/C29H25N3O7/c1-30(2)17-6-9-20-23(14-17)38-24-15-18(31(3)4)7-10-21(24)27(20)19-8-5-16(13-22(19)28(35)36)29(37)39-32-25(33)11-12-26(32)34/h5-10,13-15H,11-12H2,1-4H3

Clé InChI

VWFRSNKRTNUMET-UHFFFAOYSA-N

Application

5-Carboxy-tetramethylrhodamine N-succinimidyl ester (5-TAMRA) is used as an amine coupling reagent to form 5-carboxy-tetramethylrhodamine (5-TAMRA) derivatized compounds. 5-TAMRA derivatized compounds may be used in applications as enzyme substrates, molecular probes, molecular beacons and as fluorescence resonance energy transfer (FRET) partners.

Autres remarques

Fluorescent labeling of DNA and RNA

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)


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Consulter la Bibliothèque de documents

Simona Allocca et al.
International journal of molecular sciences, 19(7) (2018-06-30)
The H1069Q substitution is the most frequent mutation of the Cu transporter ATP7B that causes Wilson disease in the Caucasian population. ATP7B localizes to the Golgi complex in hepatocytes, but, in the presence of excessive Cu, it relocates to the
Erki Enkvist et al.
Organic & biomolecular chemistry, 10(43), 8645-8653 (2012-10-04)
Up-regulation of an acidophilic protein kinase, CK2, has been established in several types of cancer. This cognition has made CK2 an important target for drug development for cancer chemotherapy. The characterization of potential drug candidates, determination of the structure and
Angela Vaasa et al.
Analytical biochemistry, 385(1), 85-93 (2008-11-20)
The bisubstrate fluorescent probe ARC-583 (Adc-Ahx-(D-Arg)(6)-d-Lys(5-TAMRA)-NH2) and its application for the characterization of both ATP- and protein/peptide substrate-competitive inhibitors of protein kinases PKA (cyclic AMP-dependent protein kinase) and ROCK (rho kinase) in fluorescence polarization-based assay are described. High affinity of
Elke Duchardt-Ferner et al.
Nucleic acids research, 48(2), 949-961 (2019-11-23)
RNA aptamers-artificially created RNAs with high affinity and selectivity for their target ligand generated from random sequence pools-are versatile tools in the fields of biotechnology and medicine. On a more fundamental level, they also further our general understanding of RNA-ligand
D Proudnikov et al.
Nucleic acids research, 24(22), 4535-4542 (1996-11-15)
Several procedures have been described for fluorescent labeling of DNA and RNA. They are based on the introduction of aldehyde groups by partial depurination of DNA or oxidation of the 3'-terminal ribonucleoside in RNA by sodium periodate. Fluorescent labels with

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