354-05F

Human Aortic Smooth Muscle Cells: HAOSMC, fetal

• Code UNSPSC : 41106514
• Nomenclature NACRES : NA.81

Propriétés

• Source biologique: human aorta (normal, tunica intima and tunica media, fibrous plaque-free)
• Niveau de qualité: 100
• Conditionnement: pkg of 500,000 cells
• Fabricant/nom de marque: Cell Applications, Inc
• Mode de croissance: Adherent
• Caryotype: 2n = 46
• Morphologie: smooth muscle
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cardiovascular diseases
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Description générale

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Human Aortic Smooth Muscle Cells (HAOSMC) provide an excellent model system to study all aspects of cardiovascular function and disease, especially those related to mechanisms of hyperplasia and hypertrophy of intimal smooth muscle cells leading to vascular occlusion in atherosclerosis and stent restenosis.

HAOSMC have been utilized in dozens of research studies, for example, to:elucidate cytokines and growth factors signaling pathways implicated in the molecular regulation of smooth muscle cell proliferation, migration, and overall vascular function (Liu, 2009; Tan, 2009; Seymour, 2010; Chen, 2011; Hirase, 2013; Stein, 2013);

• understand hyperglycemia-related risk factors for atherosclerosis in diabetes patients (Maier, 2010), as well as effects of ethanol on vascular calcification (Oros, 2012);
• investigate extracellular elastic matrix deposition and its role in cardiovascular health, repair of damaged vasculature and successful tissue engineering (Gacchina, 2010, 2011; Venkataraman, 2012; Phillippi, 2013);
• study mechanisms and effects of mechanoregulation on proliferation and function of smooth muscle cells (Jiang, 2009; Venkataraman, 2013, 2014), and develop advanced stent technology, including novel surface materials, and drug and gene delivery systems to prevent restenosis and other occlusive vasculopathies (Brito, 2010; Deshpande, 2013; Kakade, 2013; Lamichhane, 2013).

Characterization: Positive for smooth muscle cell specific alpha-actin expression.

Origine de la lignée cellulaire

Aorta

Application

aortic wall contraction, role of smc under normal conditions, blood pressure, distribution of oxygenated blood through systemic circulation

Composants

Basal Medium containing 10% FBS & 10% DMSO

Notes préparatoires

• 3rd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
• Can be cultured at least 16 doublings

Procédure de repiquage

Please refer to the HAOSMC Culture Protocol.

Clause de non-responsabilité

Ce produit, destiné à la recherche scientifique, est soumis à une réglementation spécifique en France, y compris pour les activités d'importation et d'exportation (Article L 1211-1 alinéa 2 du Code de la Santé Publique). L'acheteur (c'est-à-dire l'utilisateur final) est tenu d'obtenir une autorisation d'importation auprès du Ministère français de la Recherche, mentionné à l'article L1245-5-1 II du Code de la Santé Publique. En commandant ce produit, vous confirmez détenir l'autorisation d'importation requise.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable