254M-1
FLI-1 (MRQ-1) Mouse Monoclonal Antibody
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About This Item
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
mouse
Niveau de qualité
100
500
Conjugué
unconjugated
Forme d'anticorps
culture supernatant
Type de produit anticorps
primary antibodies
Clone
MRQ-1, monoclonal
Description
For In Vitro Diagnostic Use in Select Regions (See Chart)
Forme
buffered aqueous solution
Espèces réactives
human
Conditionnement
vial of 0.1 mL concentrate (254M-14)
vial of 0.5 mL concentrate (254M-15)
bottle of 1.0 mL predilute (254M-17)
vial of 1.0 mL concentrate (254M-16)
bottle of 7.0 mL predilute (254M-18)
Fabricant/nom de marque
Cell Marque™
Technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:25-1:100
Isotype
IgG2b
Contrôle
pnet
Conditions d'expédition
wet ice
Température de stockage
2-8°C
Visualisation
nuclear
Informations sur le gène
human ... FLI1(2313)
Description générale
Ewing sarcoma/peripheral primitive neuroectodermal tumor (ES/PNET) is a rare primary tumor of the bone/soft tissue that resembles other undifferentiated tumors. The differential diagnosis of undifferentiated tumors of the soft tissue includes blastemal Wilms tumor, rhabdoid tumor, neuroblastoma, lymphoma, clear cell sarcoma, small cell carcinoma, synovial sarcoma (SS), neuroblastoma, desmoplastic small round cell tumor (DSRCT), and ES/PNET. It is important to correctly classify these tumors for appropriate treatment. The most common primitive renal tumor, Wilms tumor, responds well to a standard regimen of multiagent chemotherapy, whereas renal ES/PNET tends to be a high-stage, aggressive neoplasm that requires more extensive therapy .
The FLI-1 gene and FLI-1 protein are best known for their critical role in the pathogenesis of ES/PNET. More than 85% of ES/PNET are characterized by the translocation t(11;22)(q24;q12) that results in the fusion of the ews gene on chromosome 22 to the FLI-1 gene on chromosome 11. FLI-1 is a member of the ETS (erythroblastosis virus-associated transforming sequences) family of DNA-binding transcription factors and is involved in cellular proliferation and tumorigenesis. FLI-1 is normally expressed in endothelial cells and in hematopoietic cells, including T lymphocytes. The immunohistochemical detection of FLI-1 protein has been shown in two recent studies to be valuable in the discrimination of ES/PNET from most of its potential mimics, with the notable exception of lymphoblastic lymphoma.
The FLI-1 gene has also recently been shown to play an important role in the embryologic development of blood vessels. Expression of FLI-1 protein in adult endothelial cells in all types of blood vessels (arterial, venous, and lymphatic) has previously been shown both in our previous work and in that of Nilsson et al.
Folpe et al. found FLI-1 to be a highly sensitive (92%) and, with regards to the cases evaluated in this study, specific (100%) marker of both benign and malignant vascular tumors. The “absolute specificity” of FLI-1 is of course lower, given its expression in ES/PNET and lymphomas. FLI-1 expression appears to be the first reliable nuclear marker of endothelial differentiation. In particular, Folpe et al. found that FLI-1 reliably distinguished epithelioid forms of angiosarcoma from two important mimics, epithelioid sarcoma and carcinoma.
Assoc. products: WT-1, CD99, Synaptophysin, Chromogranin A, CK AE1/AE3
The FLI-1 gene and FLI-1 protein are best known for their critical role in the pathogenesis of ES/PNET. More than 85% of ES/PNET are characterized by the translocation t(11;22)(q24;q12) that results in the fusion of the ews gene on chromosome 22 to the FLI-1 gene on chromosome 11. FLI-1 is a member of the ETS (erythroblastosis virus-associated transforming sequences) family of DNA-binding transcription factors and is involved in cellular proliferation and tumorigenesis. FLI-1 is normally expressed in endothelial cells and in hematopoietic cells, including T lymphocytes. The immunohistochemical detection of FLI-1 protein has been shown in two recent studies to be valuable in the discrimination of ES/PNET from most of its potential mimics, with the notable exception of lymphoblastic lymphoma.
The FLI-1 gene has also recently been shown to play an important role in the embryologic development of blood vessels. Expression of FLI-1 protein in adult endothelial cells in all types of blood vessels (arterial, venous, and lymphatic) has previously been shown both in our previous work and in that of Nilsson et al.
Folpe et al. found FLI-1 to be a highly sensitive (92%) and, with regards to the cases evaluated in this study, specific (100%) marker of both benign and malignant vascular tumors. The “absolute specificity” of FLI-1 is of course lower, given its expression in ES/PNET and lymphomas. FLI-1 expression appears to be the first reliable nuclear marker of endothelial differentiation. In particular, Folpe et al. found that FLI-1 reliably distinguished epithelioid forms of angiosarcoma from two important mimics, epithelioid sarcoma and carcinoma.
Assoc. products: WT-1, CD99, Synaptophysin, Chromogranin A, CK AE1/AE3
Qualité
 IVD |  IVD |  IVD |  RUO |
Liaison
FLI-1 Positive Control Slides, Product No. 254S, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).
Forme physique
Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide
Notes préparatoires
Download the IFU specific to your product lot and formatNote: This requires a keycode which can be found on your packaging or product label.
Autres remarques
For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com
Informations légales
Cell Marque is a trademark of Merck KGaA, Darmstadt, Germany
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Naoto Kuroda et al.
Medical molecular morphology, 39(4), 221-225 (2006-12-26)
A 29-year-old woman presented with facial edema, and imaging disclosed a tumor extending from the anterior chest wall to the anterosuperior aspect of the mediastinum. Transbronchial cytology of the primary tumor and biopsy of the metastatic scalp lesion were performed.
P Mhawech-Fauceglia et al.
Histopathology, 49(6), 569-575 (2006-12-14)
To compare the sensitivity and specificity of the recently commercially available FLI-1 monoclonal (FLI-1m) antibody with the currently used antibodies [CD99 and FLI-1 polyclonal (FLI-1p)] in the diagnosis of Ewing's sarcoma/primitive neuroectodermal tumour (EWS/PNET) and to determine the diagnostic value
Dale A Ellison et al.
Human pathology, 38(2), 205-211 (2006-12-01)
Ewing sarcoma/peripheral primitive neuroectodermal tumor (pPNET) is a rare primary tumor of the kidney with morphologic features similar to those of other primitive tumors. Previous studies have shown that these tumors frequently stain positively with immunostains against CD99 and FLI-1
C Blind et al.
Journal of clinical pathology, 61(1), 79-83 (2007-04-07)
Archived tissue blocks preserve the antigenicity of samples for a long time under normal storage conditions, whereas tissue sections may show a diminished immunoreactivity over time. Little is known about the processes responsible for antigenicity loss and how tissue sections
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