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Merck
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Key Documents

122-20

Sigma-Aldrich

Rat Tail Collagen Coating Solution (20 ML)

50 μg/mL, solution, BioReagent, suitable for cell culture

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About This Item

Code UNSPSC :
12352202
Nomenclature NACRES :
NA.75

Stérilité

sterile; sterile-filtered

Niveau de qualité

100

Gamme de produits

BioReagent

Forme

solution

Durée de conservation

6 mo.

Conditionnement

pkg of 20 mL

Concentration

50 μg/mL

Technique(s)

cell culture | mammalian: suitable

Numéro d'accès UniProt

P02454

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Informations sur le gène

rat ... Col1a1(29393)

Description générale

Collagen type I is a fibrillar collagen that consists of two α1 chains and one α2 chain twisted together to form a triple helix structure. It is the predominant protein in vertebrates and plays a significant role in the extracellular matrices present in various tissues such as bones, tendons, skin, ligaments, cornea, and other types of connective tissue between cells. Rat Tail Collagen Coating Solution (20 ML) enhances cell attachment and proliferation, or promotion of cell-specific morphology and function. It is commonly used for endothelial cells, hepatocytes, muscle cells, and a variety of other cell types.
This collagen solution is prepared in 0.02 M Acetic Acid at 50 ug/ml and sterile filtered.

Application

Rat Tail Collagen Coating Solution (20 ML) is suitable for use in cell culture to study the growth, differentiation, and migration of cells. The rat-tail collagen I coating solution has been used to coat 96-well plates, providing a suitable surface for the adhesion and growth of primary keratinocytes in cell culture experiments.

Notes préparatoires

To prepare the tissue culture ware, apply Rat Tail Collagen Solution at a culture surface ratio of 5 ug/cm2. Here are some examples:
  • For a T-25 flask, use 125 ug (2.5 ml) of the solution.
  • For each well of a 24-well plate, use 10.5 ug (210 ul) of the solution.
After applying the solution, incubate the coated tissue culture ware at 37oC for 2 hours. Carefully remove the Collagen Coating Solution by aspiration. Rinse the coated surface twice with PBS. The coated tissue culture ware can be used immediately or air-dried and stored at 4oC for up to one week.

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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