K1637

Kanamycine sulfate from Streptomyces kanamyceticus

meets USP testing specifications, powder

• Synonyme(s) : Kanamycine sulfate salt, Kanamycine A
• Formule empirique (notation de Hill): C₁₈H₃₆N₄O₁₁ · H₂O₄S
• Numéro CAS: 25389-94-0
• Poids moléculaire : 582.58
• Numéro CE : 246-933-9
• Numéro MDL: MFCD00070253
• Code UNSPSC : 51101500
• ID de substance PubChem : 24896209
• Nomenclature NACRES : NA.76

Propriétés

• Source biologique: Streptomyces kanamyceticus
• Niveau de qualité: 200
• Agence: USP/NF; meets USP testing specifications
• Forme: powder
• Puissance: ≥750 μg per mg
• Conditions de stockage: (Tightly closed. Dry. Keep in a well -ventilated place. Keep locked up or in an area accessible )
• Solubilité: H₂O: 10-50 mg/mL (As a stock solution. Stock solutions should be stored at 2-8°C. Stable at 37°C for 5 days.)
• Spectre d'activité de l'antibiotique: Gram-negative bacteria; Gram-positive bacteria; mycobacteria; mycoplasma
• Application(s): pharmaceutical (small molecule)
• Mode d’action: protein synthesis | interferes
• Chaîne SMILES: OS(O)(=O)=O.NC[C@H]1O[C@H](O[C@@H]2[C@@H](N)C[C@@H](N)[C@H](O[C@H]3O[C@H](CO)[C@@H](O)[C@H](N)[C@H]3O)[C@H]2O)[C@H](O)[C@@H](O)[C@@H]1O
• InChI: 1S/C18H36N4O11.H2O4S/c19-2-6-10(25)12(27)13(28)18(30-6)33-16-5(21)1-4(20)15(14(16)29)32-17-11(26)8(22)9(24)7(3-23)31-17;1-5(2,3)4/h4-18,23-29H,1-3,19-22H2;(H2,1,2,3,4)/t4-,5+,6-,7-,8+,9-,10-,11-,12+,13-,14-,15+,16-,17-,18-;/m1./s1
• Clé InChI: OOYGSFOGFJDDHP-KMCOLRRFSA-N

Description

Description générale

Kanamycin Sulfate is a broad spectrum aminoglycoside-antibiotic purified from the bacterium Streptomyces kanamyceticus. Kanamycin Sulfate is a selection agent for cells in culture media and acts by binding to the 30S subunit of the bacterial ribosome which inhibits protein synthesis in susceptible bacteria.

Application

Kanamycin sulfate is used as an additive in culture media for the isolation of group D streptococci on Kanamycin Esculin Azide Agar and for selection of transformed plant cells containing the neomycin phosphotransferase on a kanamycin-medium. Kanamycin sulfate can also be used as a selection agent for cells transformed with kanamycin B resistance gene. It is recommended for use in cell culture applications at 100 μg/mL. Kanamycin sulfate from Streptomyces kanamyceticus has been used: in the research of cell type-specific expression of genes

Actions biochimiques/physiologiques

Mode d'action : Ce produit agit en se fixant à la sous-unité ribosomale 70S, ce qui inhibe la translocation et provoque des erreurs de codage.

Mode de résistance : les enzymes modifiant les aminoglycosides (dont l'acétyltransférase, la phosphotransférase et la nucléotidyltransférase) peuvent modifier cet antibiotique, l'empêchant alors d'interagir avec les ribosomes.

Spectre antimicrobien : Le sulfate de kanamycine est efficace sur les bactéries à Gram négatif, les bactéries à Gram positif et les mycoplasmes.

Caractéristiques et avantages

• High quality antibiotic suitable for mulitple research applications
• meets USP testing specifications

Notes préparatoires

Kanamycin sulfate is soluble in water at 50 mg/mL, yielding a clear solution. It is practically insoluble in alcohol, acetone, chloroform, ether and ethyl acetate. A 1% solution in water has a pH of 6.5 to 8.5. Sterile solutions can be prepared by a sterile filtration, through a .2μm filter.

Solutions are stable at 37°C for approximately 5 days. Aqueous stock solutions can be stored at 2-8°C for long term storage.

Autres remarques

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Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H360D
• Conseils de prudence: P201 - P308 + P313
• Classification des risques: Repr. 1B
• Code de la classe de stockage: 6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
• Classe de danger pour l'eau (WGK): WGK 2
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)