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114367

Sigma-Aldrich

Bromocresol Green sodium salt

ACS reagent, 90% (HPLC), Dye content 90 %

Synonyme(s) :

3′,3″,5′,5″-Tetrabromo-m-cresolsulfonphthalein sodium salt

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About This Item

Formule empirique (notation de Hill):
C21H13Br4NaO5S
Numéro CAS:
62625-32-5
Poids moléculaire :
720.00
Numéro CE :
263-657-4
Numéro MDL:
MFCD00148898
Code UNSPSC :
12171500
ID de substance PubChem :
24847167
Nomenclature NACRES :
NA.47

Qualité

ACS reagent

Niveau de qualité

200

Agence

suitable for SM 2310
suitable for SM 2320

Pureté

90% (HPLC)

Forme

powder or crystals

Composition

Dye content, 90%

visual transition interval

3.8, yellow(Visual Interval Pass)
3.8-5.2, yellow to blue
4.5, green
5.4, blue(Visual Interval Pass)

Pf

230 °C (dec.) (lit.)

Application(s)

diagnostic assay manufacturing
hematology
histology

Température de stockage

room temp

Chaîne SMILES 

[Na+].Cc1c(Br)c(O)c(Br)cc1\C(=C2\C=C(Br)C(=O)C(Br)=C2C)c3ccccc3S([O-])(=O)=O

InChI

1S/C21H14Br4O5S.Na/c1-9-12(7-14(22)19(26)17(9)24)21(13-8-15(23)20(27)18(25)10(13)2)11-5-3-4-6-16(11)31(28,29)30-21;/h3-8,26-27H,1-2H3;/q;+1/p-1

Clé InChI

HEFSGAHJDGZCHA-UHFFFAOYSA-M

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Catégories apparentées

Description générale

Bromocresol Green (BCG), also known as bromcresol green, is a synthetic, anionic, triphenylmethane dye belonging to the phthalein and sulphonphthalein dyes group. It is commonly used as a pH indicator as it changes color from yellow to blue-green at pH 3.8-5.4. The monosodium salt of bromocresol green is highly ionizable in water. The dye at a higher pH level produces a large and weakly lipophilic dianion.

Application

Bromocresol green serves as a pH indicator in biology and medicinal experiments.
  • It is used widely as a vital stain to study blood-brain permeability.
  • It is used as a tracking dye for DNA agarose gel electrophoresis, in protein determinations, and in charge-transfer complexation processes.
  • It is used in TLC for the visualization of compounds with functional groups whose pKa is below 5.0.
  • It has been used in the pH-sensitive colorimetric determination of glutamate decarboxylase activity.
  • It has also been employed in the evaluation of paper-analytical devices for colorimetric analysis of serum samples.
  • It is suitable as a selective inhibitor in kidney physiology studies.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

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Certificats d'analyse (COA)

Lot/Batch Number
SHBN5363
MKCM8710
SHBM6855
MKCL5625
MKCL9453

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Consulter la Bibliothèque de documents

A high-throughput colorimetric assay to measure the activity of glutamate decarboxylase
Yu, et al.
Enzyme and Microbial Technology, 49(3) (2011)
Regina Michelis et al.
Biochemical and biophysical research communications, 401(1), 137-142 (2010-09-14)
Hypoalbuminemia is a measure of malnutrition, inflammation and a predictor of mortality in uremia. It is controversial whether albumin levels per se are associated with the clinical outcomes in uremic patients. The co-occurrence of hypoalbuminemia and oxidative stress in hemodialysis
Fanya Ismail et al.
The Analyst, 136(4), 807-815 (2010-12-02)
In this paper we present a new theory to re-examine the immobilization technique of dye doped sol-gel films, define the strength and types of possible bonds between the immobilized molecule and sol-gel glass, and show that the immobilized molecule is
Martina O' Toole et al.
Talanta, 66(5), 1340-1344 (2008-10-31)
A novel inexpensive optical-sensing technique has been developed for colorimetric flow analysis. This sensing system employs two LEDs whereby one is used as the light source and the other as a light detector. The LED used as light detector is
N Alizadeh et al.
Die Pharmazie, 63(11), 791-795 (2008-12-17)
Rapid and sensitive spectrophotometric methods are developed for the determination of lamotrigine (LTG) in pharmaceutical dosage forms and urine samples, based on the formation of the charge-transfer (CT) complexes between LTG as an n-donor and the acceptors: bromocresol green (BCG)
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