ENTK-RO
Roche
Enterokinase
from calf intestine
Synonyme(s) :
restriction protease enterokinase
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About This Item
Produits recommandés
Source biologique
bovine (calf) intestine
Forme
lyophilized
Poids mol.
150 kDa
Conditionnement
pkg of 3 × 250 μg (11351311001)
pkg of 3 × 30 μg (11334115001)
Fabricant/nom de marque
Roche
Concentration
1:50 % (w/w)
pH optimal
8
Température de stockage
2-8°C
Description générale
Enterokinase is a protease from calf intestine and supplied in a quality optimized for the cleavage of fusion proteins.
Enterokinase is a heterodimeric serine protease involved in the conversion of trypsinogen to trypsin. It cleaves trypsinogen by highly specific manner at the trypsinogen activation peptide site following the sequence (A~p)~-L and stimulates its physiological activities.
Enterokinase is a heterodimeric serine protease involved in the conversion of trypsinogen to trypsin. It cleaves trypsinogen by highly specific manner at the trypsinogen activation peptide site following the sequence (A~p)~-L and stimulates its physiological activities.
Spécificité
Serine protease acting as a restriction protease that recognizes the amino acid sequence -(Asp)4-Lys-X. The aspartic acid residues can be partially substituted by glutamic acid.
Application
Quality
Purity: highly purified, not standardized with albumin
Quality control: function tested
Purity: highly purified, not standardized with albumin
Quality control: function tested
Enterokinase is used for the cleavage of fusion proteins at definite cleavage sites. For the processing of recombinant proteins, the desired protein is fused with Enterokinase recognition sequence. After purification of the entire fusion protein, the protein or peptide is released by incubation with enterokinase.
Notes préparatoires
Working concentration: 1:50 (w/w)
Storage conditions (working solution): A solution of enterokinase, stored at 2 to 8 °C, can be used up to one week.
Storage conditions (working solution): A solution of enterokinase, stored at 2 to 8 °C, can be used up to one week.
Autres remarques
For life science research only. Not for use in diagnostic procedures.
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Resp. Sens. 1
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
does not flash
Point d'éclair (°C)
does not flash
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Les clients ont également consulté
E R LaVallie et al.
The Journal of biological chemistry, 268(31), 23311-23317 (1993-11-05)
Enterokinase (enteropeptidase) is a heterodimeric serine protease that is responsible for the physiological activation of trypsinogen by highly specific cleavage of the trypsinogen activation peptide following the sequence (Asp)4-Lys. In this paper, we report the cloning and functional expression of
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