CK-RO
Roche
Creatine Kinase (CK)
from rabbit muscle
Synonyme(s) :
creatine phosphokinase
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About This Item
Produits recommandés
Source biologique
rabbit muscle
Niveau de qualité
Forme
lyophilized
Conditionnement
pkg of 100 mg (10127566001)
pkg of 500 mg (10736988001)
Fabricant/nom de marque
Roche
Couleur
white to light yellow
pH optimal
6.0-7.0(reverse reaction)
9.0(forward reaction)
Solubilité
water: soluble
Adéquation
suitable for immunoprecipitation
Numéro d'accès NCBI
Numéro d'accès UniProt
Application(s)
life science and biopharma
sample preparation
Activité étrangère
ATPase <0.001%
HK <0.001%
myokinase <0.001%
Température de stockage
2-8°C
Informations sur le gène
rabbit ... CKM(100009056)
Description générale
at 25 °C (800 U/mg lyophilizate at 37 °C) with creatine phosphate and ADP as the substrates, and N-acetyl-L-cysteine as the activator.
Application
Creatine kinase has been used as a component of translation buffer used in in vitro translation assay and as a component of energy regeneration mix.
Actions biochimiques/physiologiques
Creatine kinase (CK) is a guanidino-kinase that catalyzes the reversible phosphorylation of creatine to phosphocreatine at the expense of ATP. It plays crucial roles in rapid regeneration of ATP in cells with high demand for energy. Increased level of CK in human blood indicates diseases of the nervous system and the heart muscle, malignant hypothermia, and certain tumors. Metals such as Mg, Mn and Ca act as cofactors for CK. It also forms a substrate for protein kinase C, an important signaling molecule involved in cell growth. A product of enzymatic reaction of CK, phosphocreatine, is found to prevent HIV (human immunodeficiency virus) replication in macrophages.
Qualité
Contaminants: <0.001% ATPase, HK, and myokinase, each
Caractéristiques
Specific activity: Approximately 350U/mg lyophilizate at +25°C (800U/mg lyophilizate at +37°C) with creatine phosphate and ADP as the substrates, and N-acetyl-L-cysteine as activator.
Définition de l'unité
One unit CK activity (+25 °C) = 2.3 U (+37 °C), creatin-P and ADP as substrates, NAcCys as reactivator.
Forme physique
Lyophilizate, stabilized
Notes préparatoires
Storage conditions (working solution): Working solutions and storage conditions in routine applications:
Dissolve in 0.1 M imidazole buffer; aliquot and store at -15 to -25 °C.
Stable for approximately four weeks at -15 to -25 °C.
Avoid repeated freezing and thawing.
Dissolve in 0.5% NaHCO3 solution.
Do not freeze. Store protected from light.
Stable for 2 - 3 days, when stored at 2 to 8 °C.
Or
Dissolve in 30 mM glycine buffer, pH 9.0, with 0.2 mM DTT.
Stable for 2 - 3 days, when stored at 2 to 8 °C.
Dissolve in 0.1 M imidazole buffer; aliquot and store at -15 to -25 °C.
Stable for approximately four weeks at -15 to -25 °C.
Avoid repeated freezing and thawing.
Dissolve in 0.5% NaHCO3 solution.
Do not freeze. Store protected from light.
Stable for 2 - 3 days, when stored at 2 to 8 °C.
Or
Dissolve in 30 mM glycine buffer, pH 9.0, with 0.2 mM DTT.
Stable for 2 - 3 days, when stored at 2 to 8 °C.
Autres remarques
For life science research only. Not for use in diagnostic procedures.
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
does not flash
Point d'éclair (°C)
does not flash
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Les clients ont également consulté
Aurélie M Rakotondrafara et al.
Nature protocols, 6(5), 563-571 (2011-04-30)
Much of the regulation of gene expression occurs at the level of protein synthesis. In addition to the canonical translation factors, a multitude of proteins and microRNAs (miRNAs) act as regulatory trans-acting factors. Mechanistic analysis of translational control benefits from
Irina M Kuznetsova et al.
Biochimica et biophysica acta, 1596(1), 138-155 (2002-05-02)
GdmCl-induced unfolding of rabbit muscle creatine kinase, CK, has been studied by a variety of physico-chemical methods including near and far UV CD, SEC, intrinsic fluorescence (intensity, anisotropy and lifetime) as well as intensity and lifetime of bound ANS fluorescence.
J K Rao et al.
FEBS letters, 439(1-2), 133-137 (1998-12-16)
The crystal structure of rabbit muscle creatine kinase, solved at 2.35 A resolution by X-ray diffraction methods, clearly identified the active site with bound sulfates surrounded by a constellation of arginine residues. The putative binding site of creatine, which is
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