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11922416001

Roche

Anti-His6

from mouse IgG1

Synonyme(s) :

metal chelate affinity chromatography

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About This Item

Code UNSPSC :
12352203

Source biologique

mouse

Niveau de qualité

100

Conjugué

unconjugated

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

monoclonal

Forme

lyophilized

Conditionnement

pkg of 100 μg

Fabricant/nom de marque

Roche

Isotype

IgG1

Température de stockage

2-8°C

Description générale

Anti-His6 is a monoclonal antibody to His6-tagged proteins. Anti-His6 specifically recognizes an epitope of six consecutive histidine residues of both natural and recombinant proteins. The antibody reacts with native and denatured histidine-tagged fusion proteins independent of the location of the histidine-tag in the protein sequence.

Spécificité

Anti-His6 specifically recognizes an epitope of six consecutive histidine residues (His6) in natural and recombinant proteins. It reacts with both native and denatured histidine-tagged fusion proteins, independent of location of the histidine-tag in the protein sequence. In addition, Anti-His6 preferentially recognizes the C-terminal His6-epitope with high sensitivity. Anti-His6 allows specific and sensitive detection of histidine-tagged proteins irrespective of the expression system used.
The antibody recognizes an epitope of six consecutive histidine residues (His6) in natural and recombinant proteins. It reacts with both native and denatured histidine-tagged fusion proteins, independent of the epitope-sequence location.

Application

Anti-His6 is used for the detection of His6-tagged proteins in:
  • Immunoblotting (e.g., dot blots and western blots)
  • Immunoprecipitation
  • Immunoassays (ELISA)
  • Immunocytochemistry
  • Immunofluorescence

Notes préparatoires

Stabilizers: BSA
Working concentration: Working concentration of conjugate depends on application and substrate.
The following concentrations should be taken as a guideline:
  • ELISA: for detection 0.1 μg/ml; for coating 1 to 5 μg/ml
  • Immunoprecipitation: 0.5 to 5 μg/ml
  • Western blot: 0.2 to 0.5 μg/ml

Reconstitution

Add 1 ml double-distilled water to a final concentration of 100 μg/ml.

Autres remarques

For life science research only. Not for use in diagnostic procedures.

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Pictogrammes

Exclamation mark
GHS07

Mention d'avertissement

Warning

Mentions de danger

H317,H412

Classification des risques

Aquatic Chronic 3 - Skin Sens. 1

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

does not flash

Point d'éclair (°C)

does not flash

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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