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11418165001

Roche

Biotin Protein Labeling Kit

sufficient for 5 labeling reactions, kit of 1 (5 components), suitable for immunoblotting, suitable for ELISA

Synonyme(s) :

Biotin, protein labeling

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About This Item

Code UNSPSC :
12352200

Utilisation

sufficient for 5 labeling reactions

Niveau de qualité

100

Conditionnement

kit of 1 (5 components)

Fabricant/nom de marque

Roche

Technique(s)

ELISA: suitable
immunoblotting: suitable

Température de stockage

2-8°C

Description générale

For labeling of proteins with biotin.
Free amino acids of the protein to be labeled react with D-biotinoyl-ε-aminocaproic acid-N-hydroxy-succinimide ester (biotin-7-NHS) by forming a stable amino bond. Non reacted biotin-7-NHS is separated on a Sephadex G-25 column.

Application

Biotin protein labeling kit has been used to produce biotinylated denatured bovine serum albumin (d-BSA) for coating cadmium telluride (CdTe) quantum dot (QD) conjugates.
The kit is suitable for the labeling of proteins with biotin for immunochemistry, immunoblotting, and ELISA.

Conditionnement

1 kit containing 5 components.

Qualité

Function tested.

Notes préparatoires

Working solution: Preparation of the solutions
  • Blocking solution: Dissolve the contents of the bottle blocking reagent (green screw cap) in 300 ml double-distilled water.
  • PBS solution: Dissolve the contents of the bottle PBS (blue screw cap) in 1 I double-distilled water.
  • Biotin-7-NHS solution: The solution should be freshly prepared before labeling. Add to the contents of the reaction vial which includes biotin-7-NHS 250 μI DMSO and vortex the solution several times. The resulting concentration of the biotin-7-NHS solution is 20 mg/ml. For the labeling mix, the volume of the biotin-7-NHS solution should not exceed 5% of the total reaction volume (e.g., add maximal 50 μl biotin-7-NHS solution to 1 ml protein solution).

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Composants de kit seuls

Réf. du produit
Description
  • Blocking Reagent (green screw cap)
  • Phosphate Buffered Saline (PBS) (blue screw cap)
  • D-Biotinoyl-ε-aminocaproic acid-N-hydroxysuccinimide ester (Biotin-7-NHS)
  • Dimethylsulfoxide (DMSO) (red screw cap)
  • Sephadex G-25 Columns

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Certificats d'analyse (COA)

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Peng C, et al.
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Immunotherapy for ovarian cancer is an area of intense investigation since the majority of women with relapsed disease develop resistance to conventional cytotoxic therapy. The paucity of safe and validated target antigens has limited the development of clinically relevant antibody-based
Xing Wei et al.
Stem cells (Dayton, Ohio), 27(2), 478-488 (2008-11-22)
Adipose tissue stroma contains a population of mesenchymal stem cells, which support repair when administered to damaged tissues, in large part through secreted trophic factors. We directly tested the ability of media collected from cultured adipose-derived stem cells (ASCs) to
Peter Bults et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1144, 122079-122079 (2020-04-05)
The quantitative determination of intact proteins in biological samples by LC with high-resolution MS detection can be a useful alternative to ligand-binding assays or LC-MS-based quantification of a surrogate peptide after protein digestion. The 22-kDa biopharmaceutical protein somatropin (recombinant human

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